Pour Your Own DNA Electrophoresis Workflow

Electrophoresis reagents optimized for success

When pouring your own agarose gels, choosing high-quality agarose, smarter-sized DNA ladders, and high-sensitivity DNA stains can help you achieve optimal electrophoresis results.

Agarose gels

Agarose gels are used for DNA fragment separation and analysis. For gel preparation you will need agarose powder and electrophoresis running buffer.

UltraPure Low Melting Point Agarose is recommended for in-gel enzymatic reactions and rapid DNA gel extraction protocols.

Buffers

Buffers are ionic solutions with electrical conductivity that preserve the pH and enable nucleic acid mobility during electrophoresis.

UltraPure 10X TBE Buffer is ideal for small DNA fragment (<1,000 bp) analysis. TBE buffers have a higher buffering capacity allowing it to be used for prolonged electrophoresis runs.

UltraPure 10X TAE Buffer is ideal for longer than 1,000 bp DNA fragment analysis and downstream enzymatic applications such as cloning.

UltraPure DNase/RNase-Free Distilled Water is designed for use in all sensitive molecular biology applications.

For easy loading and tracking of DNA samples on agarose gels.

6X TrackIt Cyan/Orange is designed for tracking of DNA fragments as small as 50 bp as it contains xylene cyanol and orange G tracking dyes.

6X TrackIt Cyan/Yellow is designed for tracking of DNA fragments as small as 10 bp as it contains xylene cyanol and tartrazine.

Invitrogen DNA ladders are available in a wide variety of size ranges (10 bp to 15 kb) and formats for different applications. The proprietary manufacturing produces high quality products that can be stored at room temperature.

Invitrogen TrackIt DNA Ladders are designed for use with agarose gels and are provided in convenient, ready-to-load formats.

Invitrogen Mass DNA Ladders are designed for accurate quantification and sizing of DNA fragments in agarose gels.

Invitrogen DNA Ladders are provided with the loading buffer in a separate tube.

Invitrogen E-Gel DNA Ladders are formulated specifically for maximum performance on Invitrogen E-Gel precast agarose gels and are provided in ready-to-load formats.

DNA staining dyes are used for visualizing the nucleic acid fragments under blue or UV light.

Invitrogen SYBR Safe DNA Gel Stain is a safer alternative to ethidium bromide for DNA band visualization on your agarose gels.

SYBR Gold Nucleic Acid Gel Stain is the most sensitive staining dye for the visualization of DNA fragment in concentrations as low as 0.025 ng/band.

UltraPure Ethidium Bromide is the standard staining dye which allows the visualization of DNA in concentrations as low as 1 ng/band.

To perform electrophoresis, a power supply, electrophoresis tank, and other accessories are required.

Owl Electrophoresis Systems enable fast agarose gel electrophoresis of nucleic acids and proteins using tanks, chambers, casters, plates, spacers, combs, power supplies, and other accessories.

E-Gel Precast Agarose Gel Systems deliver fast, bufferless agarose electrophoresis with ready-to-use precast agarose cassettes and in-gel stain. This represents an ideal system for analyzing PCR products, restriction digests and plasmid preparations.

Safe Imager 2.0 Blue Light Transilluminator is designed for viewing stained gels on the laboratory benchtop, or within a gel documentation system.

Agarose gels

Agarose gels are used for DNA fragment separation and analysis. For gel preparation you will need agarose powder and electrophoresis running buffer.

UltraPure Low Melting Point Agarose is recommended for in-gel enzymatic reactions and rapid DNA gel extraction protocols.

Buffers

Buffers are ionic solutions with electrical conductivity that preserve the pH and enable nucleic acid mobility during electrophoresis.

UltraPure 10X TBE Buffer is ideal for small DNA fragment (<1,000 bp) analysis. TBE buffers have a higher buffering capacity allowing it to be used for prolonged electrophoresis runs.

UltraPure 10X TAE Buffer is ideal for longer than 1,000 bp DNA fragment analysis and downstream enzymatic applications such as cloning.

UltraPure DNase/RNase-Free Distilled Water is designed for use in all sensitive molecular biology applications.

For easy loading and tracking of DNA samples on agarose gels.

6X TrackIt Cyan/Orange is designed for tracking of DNA fragments as small as 50 bp as it contains xylene cyanol and orange G tracking dyes.

6X TrackIt Cyan/Yellow is designed for tracking of DNA fragments as small as 10 bp as it contains xylene cyanol and tartrazine.

Invitrogen DNA ladders are available in a wide variety of size ranges (10 bp to 15 kb) and formats for different applications. The proprietary manufacturing produces high quality products that can be stored at room temperature.

Invitrogen TrackIt DNA Ladders are designed for use with agarose gels and are provided in convenient, ready-to-load formats.

Invitrogen Mass DNA Ladders are designed for accurate quantification and sizing of DNA fragments in agarose gels.

Invitrogen DNA Ladders are provided with the loading buffer in a separate tube.

Invitrogen E-Gel DNA Ladders are formulated specifically for maximum performance on Invitrogen E-Gel precast agarose gels and are provided in ready-to-load formats.

DNA staining dyes are used for visualizing the nucleic acid fragments under blue or UV light.

Invitrogen SYBR Safe DNA Gel Stain is a safer alternative to ethidium bromide for DNA band visualization on your agarose gels.

SYBR Gold Nucleic Acid Gel Stain is the most sensitive staining dye for the visualization of DNA fragment in concentrations as low as 0.025 ng/band.

UltraPure Ethidium Bromide is the standard staining dye which allows the visualization of DNA in concentrations as low as 1 ng/band.

To perform electrophoresis, a power supply, electrophoresis tank, and other accessories are required.

Owl Electrophoresis Systems enable fast agarose gel electrophoresis of nucleic acids and proteins using tanks, chambers, casters, plates, spacers, combs, power supplies, and other accessories.

E-Gel Precast Agarose Gel Systems deliver fast, bufferless agarose electrophoresis with ready-to-use precast agarose cassettes and in-gel stain. This represents an ideal system for analyzing PCR products, restriction digests and plasmid preparations.

Safe Imager 2.0 Blue Light Transilluminator is designed for viewing stained gels on the laboratory benchtop, or within a gel documentation system.