In biolaboratories, agarose gel electrophoresis is the modus operandi for size-based separation of DNA and RNA fragments. The agarose gel matrix is porous and acts as a sieve through which the nucleic acid molecules migrate. To maximize accuracy of DNA resolution, it is essential to choose high-quality agarose, smart-sized DNA ladders, and high-sensitivity DNA stains. Thermo Fisher Scientific offers a wide range of reagents optimized for agarose gel electrophoresis.
Agarose is the basic ingredient for casting gels and is available in powder and tablet forms. Agarose is dissolved in boiling water; upon cooling, agarose transitions from a liquid to gel.
Traditionally, agarose powder has been used for manually casting gels. UltraPure Agarose powder is available in three formulations to suit various agarose gel electrophoresis applications:
Agarose tablets are a more convenient format to use compared to traditional agarose powder. The benefits of TopVision agarose tablets include:
E-Gel Precast Agarose Gel Systems offer the convenience of bufferless agarose gel electrophoresis with ready-to-use precast agarose gel cassettes containing in-gel stain. The E-Gel Precast Agarose Gel Systems are suitable for both standard and high throughput applications.
Buffers are ionic solutions with electrical conductivity that preserve the pH and facilitate nucleic acid mobility during electrophoresis.
Loading buffers are used to load sample DNA and ladders into the gel wells. The loading dyes contain chromogenic agents that aids visualization of DNA migration in a gel.
Invitrogen DNA ladders are available in a wide assortment of sizes ranging between 10 bp and 15 kb. The proprietary manufacturing processes produce high purity ladder fragments. These ladders are suitable for use in agarose gels and can be stored at room temperature.
DNA staining dyes are used for visualizing and imaging the nucleic acid fragments under blue or UV light. The staining dyes bind to the DNA molecule and fluoresce under UV light.
In addition to high-quality biochemicals, dependable devices are essential for performing agarose gel electrophoresis. The devices required for agarose gel electrophoresis include gel casting and gel running systems, power supply devices, and transilluminators.
Owl Horizontal Electrophoresis Systems are gel casting and gel running systems for high-, low-, and standard-throughput electrophoresis. The horizontal systems are recommended for agarose gel electrophoresis and are available in multiple formats including, large, micro, wide, and mini gels. Accessories such as tanks, chambers, casters, plates, spacers, and combs are also available.
Owl electrophoresis systems can be tailored to fit your needs.
Use the Safe Imager 2.0 Blue Light Transilluminator for safely viewing stained gels on the laboratory benchtop, or within a gel documentation system.
PowerEase Touch Power Supplies bring a new level of convenience to your electrophoresis experiments. With a bright LCD touchscreen interface, you can enter in custom programs or use the preprogrammed protein gel protocols.
Nucleic acid electrophoresis products
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Nucleic acid electrophoresis education
Learn about gel electrophoresis basics, workflow, considerations, applications, and troubleshooting for separation and analysis of nucleic acids.
DNA and RNA protocols
Find easy-to-follow protocols for everyday experiments, including agarose protocols and mRNA protocols.
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