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Agarose Gel Electrophoresis: Devices & Reagents |
Agarose gel electrophoresis a standard laboratory technique for size-based separation of DNA and RNA fragments. The agarose gel matrix is porous and acts as a sieve through which the nucleic acid molecules migrate according to relative size. To maximize effectiveness of DNA separation, it is essential to choose high-quality agarose, chromatographically purified DNA ladders, and high-sensitivity DNA stains. We offer a wide range of reagents optimized for agarose gel electrophoresis.
Agarose is the basic ingredient for casting gels and is available in powder and tablet forms.
Traditionally, agarose powder has been used for manually casting gels. UltraPure Agarose powder is available in three formulations to suit various agarose gel electrophoresis applications:
E-Gel Precast Agarose Gel Systems offer the convenience of buffer-less agarose gel electrophoresis with ready-to-use precast agarose gel cassettes containing in-gel stain. The E-Gel Precast Agarose Gel Systems are suitable for both standard and high throughput applications.
Reagents for agarose gel electrophoresis, such as running buffers, loading buffers, DNA ladders, and DNA stains, are essential for the separation and visualization of DNA fragments. These components help ensure optimal migration of DNA through the gel matrix, provide reference markers for fragment size determination, and help enable the detection of DNA bands under UV light or other imaging systems.
Buffers are ionic solutions with electrical conductivity that preserve the pH and facilitate nucleic acid mobility during electrophoresis.
Loading buffers are used to load sample DNA and ladders into the gel wells. The loading dyes contain chromogenic agents that aid visualization of DNA migration in a gel.
Invitrogen DNA ladders are available in a wide assortment of sizes ranging between 10 bp and 15 kb. The proprietary manufacturing processes produce high purity ladder fragments. These ladders are suitable for use in agarose gels and can be stored at room temperature.
DNA staining dyes are used for visualizing and imaging the nucleic acid fragments under blue or UV light. The staining dyes bind to the DNA molecule and fluoresce under UV light.
In addition to high-quality biochemicals, dependable devices are essential for performing agarose gel electrophoresis. The devices required for agarose gel electrophoresis include gel casting and gel running systems, power supply devices, and transilluminators.
Owl Horizontal Electrophoresis Systems are agarose gel casting and running systems for high-, low-, and standard-throughput electrophoresis. The horizontal systems are recommended for agarose gel electrophoresis and are available in multiple formats including, large, micro, wide, and mini gels. Accessories such as tanks, chambers, casters, plates, spacers, and combs are also available.
Owl electrophoresis systems can be tailored to fit your needs. Learn more about custom Owl systems
Use the Safe Imager 2.0 Blue Light Transilluminator to safely view stained gels on the laboratory benchtop or within a gel documentation system.
PowerEase Touch Power Supplies bring a new level of convenience to your electrophoresis experiments. With a bright LCD touchscreen interface, you can enter in custom programs or use the pre-programmed gel electrophoresis protocols.
Access learning resources—a knowledge base for all things nucleic acid electrophoresis.
View our webinars to learn more about molecular biology topics.
E-Gel Precast Gel and Ladder Selection Tool for Invitrogen Power Snap devices.
Find answers to your questions on nucleic acid gel electrophoresis using acrylamide or agarose gels, including E-gels.
For Research Use Only. Not for use in diagnostic procedures.
Nucleic acid electrophoresis products
Explore Thermo Fisher Scientific’s suite of products for all your electrophoresis needs.
Nucleic acid electrophoresis education
Learn about gel electrophoresis basics, workflow, considerations, applications, and troubleshooting for separation and analysis of nucleic acids.
DNA and RNA protocols
Find easy-to-follow protocols for everyday experiments, including agarose protocols and mRNA protocols.
Nucleic acid purification and analysis support center
Find tips, troubleshooting help, and resources for your nucleic acid purification and analysis applications.
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For additional questions, reach out to our Technical Application Scientists.