Invitrogen DNA Ladders are available for a wide variety of size ranges, applications, and formats. To create our DNA Ladders, each fragment is purified individually using proprietary chromatography-based technology, resulting in DNA Ladders of superior quality which exceeds industry standards. DNA Ladders are stable during prolonged incubation at room temperature and multiple freeze-thaw cycles.
New and improved Invitrogen DNA Ladders─released September, 2017
- Chromatography-purified DNA fragments for brighter, sharper bands
- Optimized size ranges for easier analysis
- Improved loading dyes for clear tracking and analysis
The key to accurate band analysis is to use the correct DNA ladder for your particular application. Use the Invitrogen DNA Ladder selection table below to help you choose the DNA ladder you need.
- Invitrogen E-Gel DNA Ladders are formulated specifically for maximum performance on Invitrogen E-Gel precast agarose gels and are provided in ready-to-load formats.
- Invitrogen TrackIt DNA Ladders are designed for use with agarose gels and are provided in convenient, ready-to-load formats.
- Invitrogen Mass DNA Ladders are designed for accurate quantification and sizing of DNA fragments in agarose gels.
- Invitrogen DNA Ladders are provided with the loading buffer in a separate tube.
Table 1. Invitrogen DNA Ladder selection guide
|Product||Range, base pair (bp)||Chromatography-purified fragments||Ready to load||Loading dyes or buffer supplied|
|Invitrogen E-Gel DNA Ladders||10 to 15,000||Yes||Yes||Xylene Cyanol, Tartrazine (1X E-Gel Sample Loading Dye) or Xylene Cyanol, Orange G (1X E-Gel Sample Loading Dye)|
|Invitrogen TrackIt DNA Ladders||1,000 to 10,000 (High mass)
100 to 2,000 (Low mass)
|Yes||Yes||TrackIt Cyan/Yellow Loading dye, TrackItCyan/Orange Loading Dye|
|Invitrogen Mass DNA Ladders||10 to 15,000||Yes||No||Provided with 10X BlueJuice Gel Loading Buffer|
|Invitrogen DNA Ladders||10 to 15,000||Yes||No||Provided with 10X BlueJuice Gel Loading Buffer|
Ambient shipping helps to reduce environmental footprint
To reduce the annual carbon footprint associated with packaging and shipping our DNA Ladders, we use ambient (non-insulated) shipping with many of our DNA Ladders. Products approved for shipping at ambient temperatures have undergone rigorous testing to ensure ambient shipping has no impact on their quality and stability.
Benefits of chromatography-based purification of DNA Ladder fragments
The chromatography-based purification method results in exceptional purity and quality of DNA fragments used to make Invitrogen DNA Ladders. This method also enables precise in-gel quantification because the concentration of each DNA fragment in the DNA Ladders is determined spectrophotometrically. In addition, the chromatography-based purification method does not require the use of organic solvents, making it a more environmentally friendly process.
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