The 100 bp DNA Ladder is suitable for sizing double-stranded DNA from 100 to 1,500 bp. Prepared from a plasmid containing repeats of a 100 bp DNA sequence, the ladder consists of 15 blunt-ended fragments ranging in length from 100 to 1,500 bp, in 100 bp increments, and an additional fragment at 2,072 bp. This ladder is ideal for determining the size of PCR products.

Easy Visual Reference On Agarose Gels

  The double-stranded ladder can be visualized on 1%–2% agarose gels after staining with ethidium bromide or SYBR® Safe stain. For easy visual reference on agarose gels, the 600 bp band is 2–3 times brighter than the other bands in the ladder. This ladder can be radiolabeled using T4 polynucleotide kinase or T4 DNA polymerase.

Figure 1 (right). 3 µg/lane; 1.5% agarose gel stained with ethidium bromide.