In gel electrophoresis, fluorescent nucleic acid gel stains are dyes used for DNA detection. When bound to DNA molecules and illuminated with UV or blue light, these dye molecules fluoresce brightly. Once the gel is stained, transilluminators are used to excite the dye molecules, which allow DNA to be visualized, sized, and quantified. The SYBR family of dyes is a safer and more sensitive alternative to ethidium bromide (EtBr).
|UltraPure Ethidium Bromide
|SYBR Safe DNA Gel Stain
|SYBR Green I Nucleic Acid Gel Stain
|SYBR Gold Nucleic Acid Gel Stain
|Detection of nucleic acids in agarose gels and CsCl gradients
|Safer, smarter alternative to EtBr
|Ultimate sensitivity for capillary electrophoresis and real-time PCR
|Ultimate sensitivity for DNA detection
|Sensitivity with dsDNA
|Available as ready-to-use
|Recommended for gel electrophoresis
|Recommended for ssDNA
|Improved cloning efficiency; avoid UV-induced nicked DNA
|UV or blue light
|Fluorescence (blue light excitation)
|UV, blue light, or laser scanners
SYBR Gold stain is a versatile and universal nucleic acid gel stain that provides excellent sensitivity for dsDNA, ssDNA, and RNA detection. SYBR Gold stain can be used with many gel types and does not require a wash step.
SYBR Gold stain is an excellent choice for use in molecular biology applications such as northern blotting, denaturing gradient gel electrophoresis, and single-strand conformation polymorphism, as well as routine gel analysis.
SYBR Gold stain offers multiple benefits when compared to other post-electrophoresis stains, including:
SYBR Green I Nucleic Acid Gel Stain is an extremely sensitive fluorescent stain for detecting nucleic acids in both agarose and polyacrylamide gels, detecting as little as 60 pg of DNA. SYBR Green stains and SYBR Gold stains share many similarities, but SYBR Green stains are slightly less sensitive than SYBR Gold stain.
SYBR Green I stain has some important qualities, making it the preferred reagent for certain applications.
SYBR Safe DNA gel stain is the safest reagent for staining DNA. SYBR Safe stain has sensitivity comparable to that of EtBr, yet tests negative in three mammalian cell-based assays for genotoxicity. It is less mutagenic than ethidium bromide in standard Ames tests and is not classified as hazardous waste under U.S. federal regulations.
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For Research Use Only. Not for use in diagnostic procedures.