E-Gel High Throughput DNA Electrophoresis | Thermo Fisher Scientific

Automatically run 48 to 96 samples per gel and more

Turn routine agarose gel electrophoresis into an automated, high-throughput operation with E-Gel 48 or 96 gels and E-Base integrated power systems. Whether you are analyzing multiple PCR products, plasmid preparations, or restriction digests or are performing SNP analysis, there is an E-Gel precast agarose gel for your throughput needs.

Integrated system with small footprint
Well format compatible with most robotic loading systems
Bar-coded cassettes for easy tracking

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E-Base Integrated Power System

E-Gel 48 and E-Gel 96 gels run on a specially designed, space-saving system of Mother and Daughter E-Bases – each a combined gel base and power supply all in one. These integrated, all-inclusive power supplies have a small bench-top footprint measuring only 5.5 x 6 inches per base, and can be plugged directly into any standard electric outlet.

The Mother E-Base has an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-Gel 48, E-Gel 96, or E-PAGE 96 gels.

Each Mother E-Base has a pwr/prg (power/program) button (right side) and a time button (left side) on the lower right side of the base. The lower left side of each Mother E-Base contains a light LED and a digital display. The gel cassette is inserted into the two electrode connections. The Mother E-Base is connected to an electrical outlet with the electrical plug.

The Daughter E-Base connects to the Mother E-Base, and together they can be used for the electrophoresis of two or more, E-Gel 48, E-Gel 96, or E-PAGE 96 gels.

Connect multiple Daughter E-Bases to the Mother E-base to create a multi-units system capable of running four gels at once. Each base has a lighted LED, digital timer display, and power/program and timer button to indicate and control the electrophoresis process. Pre-set programs include a 12 minute DNA program for running E-Gel 96 gels and a 14-minute protein run program for running E-PAGE 96 gels. An extended 23 minute runtime is used to run E-Gel 48 gels.

Compatible E-Gel precast gels

E-Gel 48 gels

E-Gel 48 gels have two rows, each containing 24 sample and 2 marker wells with a 3.2 cm run length. The 4% E-Gel 48 gels are made with high-resolution agarose for clear band resolution and distinct band separation. Loading E-Gel 48 gels is fast and simple with a multichannel pipettor, or use robotic liquid handling systems for increased throughput. E-Gel 48 gels run in E-Base devices in just 20 minutes.

E-Gel 96 gels

E-Gel 96 gels contain 96 sample lanes and 8 marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp to 10 kb. The staggered-well format is compatible with 8-, 12-, or 96-tip robotic loading systems. Band identification and tracking are effortless, with fluorescent lane numbers that instantly transfer during photodocumentation. E-Gel 96 gels run in E-Base devices in just 12 minutes.

E-Holder Platform

The E-Holder platform is designed with a spring-loaded mechanism so that the E-Gel 96 and E-Gel 48 gel cassettes stay firmly in place during robotic loading. This allows reproducible placement and loading from one gel to the next without having to make adjustments to the robot loading software. In addition, the E-Holder platform allows you to load samples on one E-Gel 96 or E-Gel 48 gel while other gels are running in the Mother or Daughter E-Base combination.

E-Editor 2.0 Software

Analysis of E-Gel 48 and 96 results is fast and easy using E-Editor 2.0 software. E-Editor 2.0 software takes the digital image of your gel and reconfigures the lanes into one regrouped and aligned image for easy comparison, documentation, and reference back to the original plate coordinates, for quick identification of important results. The E-Editor 2.0 software is available free of charge with the purchase of E-Gel 48 and 96 gels and related equipment.

Download E-Editor 2.0 Software

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Resources

Have questions about the E-Gel system or new to molecular biology applications? Access literature, selection guides, webinars, and additional educational resources below for more information.