Safe Imager™ 2.0 Blue Light Transilluminator

Eliminate safety concerns and minimize damage to your samples

  • Does not cause burn to your skin and eyes
  • Minimizes damage to your DNA samples and gives enhanced cloning efficiencies

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About the Safe Imager™ 2.0 Blue Light Transilluminator

Enjoy superior performance and versatility

  • Electrical power cord includes adapter for connecting inside most gel documentation systems
  • Produces brighter light and more uniform emission than conventional blue light sources
  • Provides optimal excitation for SYBR® Safe DNA gel stain
  • Optimized for use with other nucleic acid and protein stains such SYBR® Gold, SYBR® Green I & II, SYPRO® Ruby, SYPRO® Orange, and Coomassie Fluor™ Orange stains


Instrument Specifications

  • Instrument dimensions: 195 × 325 × 65 mm (11.6 × 12.8 × 2.6 in)
  • Viewing surface dimensions: 190 × 190 mm (7.5 × 7.5 in)
  • LED life: 50,000 hours 
  • Complies with the European Community Safety requirements
  • This device contains Class 1 LED products
  • Light source: light emitting diodes (LED) producing a narrow emission peak centered at ~470 nm
  • Included accessories: amber filter unit, viewing glasses, and international power cord

Increase Cloning Efficiency and Reduce DNA Damage

Difference in cloning efficiency with PCR products visualized under UV light verse blue light Figure 1. Even a 30-second exposure to ethidium bromide and UV reduces cloning efficiency.  Gels loaded with equal amounts of a PCR product (1.25 kb gene fragment from Ultimate™ ORF IOH# 11050) were stained with either SYBR® Safe DNA gel stain (1:10,000 in TBE) or ethidium bromide (0.5 µg/ml in TBE) following electrophoresis. The gel stained with SYBR® Safe stain was visualized on a blue-light box with light emission identical to that produced by the Safe Imager™ transilluminator (S37102). The ethidium bromide-stained gel was visualized using UV transillumination. Bands were excised at defined exposure times. DNA was purified from the gel fragments under identical conditions and used in parallel sub-cloning reactions. Following transformation into OneShot® TOP 10 chemically competent bacteria, three serial dilutions were plated and colonies counted using an Alpha Innotech imaging system. A plot of the experiment is shown here.

Figure 2. Use of SYBR® Safe Stain and the Safe Imager™ Transilluminator causes minimal DNA damage.
Equivalent fractions of supercoiled DNA stained with SYBR® Safe DNA Gel Stain or ethidium bromide were exposed to blue light (Safe Imager™ Transilluminator, Invitrogen) (A) or UV light (B), respectively, for defined periods of time and evaluated by agarose electrophoresis. A slowermigrating species is indicative of a linear or relaxed circular vector resulting from DNA nicking or strand breaks.