Ambion™ RNA Century™-Plus Markers comprise a set of 7 in vitro–generated transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases. The Century-Plus Markers and Century™ Marker Templates are ideal for use in isotopic and nonisotopic ribonuclease protection assays and when single-stranded RNA size standards are needed for denaturing gel electrophoresis.
The Century™ Marker Templates contain mixtures of linearized plasmids ready for use as templates in in vitro transcription reactions for synthesis of labeled RNA molecular size standards. When transcribed with T7 RNA polymerase, Century™ Marker Templates produce 5 RNA transcripts of 100, 200, 300, 400, and 500 bases. Century™-Plus Marker Templates produce 7 RNA transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases.
Figure 1. RNA Century-Plus Markers (2 µg), along with an RNA transcript, were electrophoresed on an 8 M urea/5% polyacrylamide gel and stained with ethidium bromide.
|DNA Ladders||Base Pair (bp) Range|
|RNA Century-Plus Markers||100–1,000 bases|
|Century Marker Templates||100–500 bases|
|Century-Plus Marker Templates||100–1,000 bases|
For Research Use Only. Not for use in diagnostic procedures.