RNA isolation is a crucial step in the journey to discovery. For over two decades, our scientists have developed innovative and robust RNA extraction technologies to make those steps faster and more reliable.
What is the purpose of RNA extraction?
The purpose of RNA extraction is to obtain high quality purified RNA from biological samples for applications such as sequencing, transcriptome analysis, and infectious pathogen testing.
Obtaining high quality RNA is the first, and often the most critical, step in performing many RNA-based molecular techniques, such as RT- and qRT-PCR, dPCR, RNA sequencing, array analysis, northern analysis, and cDNA library construction.
There are several common methods for RNA extraction available as kits. The choice of RNA extraction kit or RNA isolation kit depends on your sample type, throughput required, or what equipment is available in the lab.
RNA extraction and isolation methods comparison chart
|Organic extraction||Crude lysate||Silica spin column||Magnetic beads|
|Description||Monophasic solution of phenol and guanidine isothiocyanate||Lysis buffer containing enzymes and detergent||Spin column and reagents for RNA isolation via selective silica binding||Magnetic beads and reagents for RNA isolation via selective bead binding|
|Sample type(s)||Bacteria, blood, cells, plant samples, tissue, viral samples, yeast||Cells in culture||Bacteria, blood, cells, plant and animal tissue, serum, yeast||Flexible for many sample types|
|Procedure||RNA isolation achieved through organic extraction and RNA precipitation||Incubation in lysis buffer followed by addition of stop solution; no isolation is performed||Homogenized samples loaded onto column; RNA is washed and eluted off column using benchtop centrifuge or vacuum manifold||Homogenized samples mixed with magnetic beads; beads are washed with wash buffers, and RNA is then eluted off the beads|
|Throughput||Low||Medium to high||Medium to high||Medium to high|
Find the right RNA extraction kit or reagent to support your research
Whatever your sample type, RNA type or downstream application, you can be confident that our portfolio of products can accelerate your research.
Need bulk reagents or a custom configuration for your workflow? Just ask our RNA Custom Services team!
Popular RNA isolation kits, reagents, and supplies
Resources and support for RNA extraction kits and RNA isolation reagents
For Research Use Only. Not for use in diagnostic procedures.