Nuclease contamination in reagents used for nucleic acid isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure. In trying to pinpoint the sources of contamination, it is easy to overlook the water used either to prepare reagents or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. We offer several brands and grades of nuclease-free water—diethylpyrocarbonate (DEPC)-treated water, nuclease-free water (not DEPC-treated), and RT-PCR grade water—that all have been rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
We offer several brands and molecular grades of nuclease-free water.
|Nuclease-free water||Invitrogen Nuclease-Free Water|
|Invitrogen UltraPure DNase⁄RNase-Free Distilled Water|
|Invitrogen DEPC-Treated Water|
|Invitrogen UltraPure DEPC-Treated Water|
|RT-PCR Grade Water|
|Invitrogen RT-PCR Grade Water|
To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.
For Research Use Only. Not for use in diagnostic procedures.