Nuclease-Free Water

Molecular grade nuclease-free water

Nuclease contamination in reagents used for nucleic acid isolation and analysis can contribute to experimental inconsistency and sometimes even experimental failure. In trying to pinpoint the sources of contamination, it is easy to overlook the water used either to prepare reagents or to resuspend precipitated RNA. Even purified water can have a high pH and minerals that can interfere with reactions that require specific salt and pH conditions. We offer several brands and grades of nuclease-free water—diethylpyrocarbonate (DEPC)-treated water, nuclease-free water (not DEPC-treated), and RT-PCR grade water—that all have been rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

We offer several brands and molecular grades of nuclease-free water.

Selection guide for nuclease-free water

Function	Product	Description
Nuclease-free water	Invitrogen Nuclease-Free Water	Not DEPC-treated Autoclaved, membrane-filtered RNase- and DNase-free Ideal for applications that may be acutely sensitive to residual DEPC (i.e., oocyte injection)
	Invitrogen UltraPure DNase⁄RNase-Free Distilled Water	Membrane-filtered Protease-, RNase-, and DNase-free Designed for use in all molecular applications
	DEPC-Treated Water
	Invitrogen DEPC-Treated Water	Autoclaved, membrane-filtered RNase- and DNase-free Autoclaved before and after packaging
	Invitrogen UltraPure DEPC-Treated Water	Prepared by incubating with 0.1% diethylpyrocarbonate (DEPC) Autoclaved to remove the DEPC; sterile filtered RNase- and DNase-free Autoclaved before and after packaging
	RT-PCR Grade Water
	Invitrogen RT-PCR Grade Water	PCR-grade water Each lot tested for nuclease contamination Autoclaved, membrane-filtered RNase- and DNase-free, genomic DNA-free Ideal for use in any PCR or RT-PCR application

To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.

Molecular grade nuclease-free water

Selection guide for nuclease-free water

Ordering information

DEPC-Treated Water

Nuclease-Free Water (not DEPC-treated)

RT-PCR Grade Water

UltraPure DNAse/RNase-Free Distilled Water