rna-stabilization-showcase

Obtaining high-quality, intact RNA is the first, and often most critical, step in performing gene expression analysis. In order to isolate high-quality RNA, the tissue has to be either: a) processed immediately after harvest, b) snap-frozen, or c) stabilized in an intermediary solution to preserve RNA integrity and allow for storage. We offer several products designed specifically to stabilize and preserve the integrity of RNA either at the point of collection or even post-collection: RNAlater Tissue Collection: RNA Stabilization SolutionRNAlater-ICE Frozen Tissue Transition Solution, and THE RNA Storage Solution.

Stabilize your samples

Product TRIzol Reagent
RNAlater Stabilization Solution
Description Aqueous, nontoxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA
Sample Types
  • Animal tissues
  • Cells
  • Tissue culture cells
  • E. coli
  • Drosophophila
  • White blood cells
  • Plant tissues
Product Sizes 100 mL, 500 mL, 50 x 1.5 mL, 20 x 5 mL, 1 x 250 mL
Protocol RNAlater Tissue Collection: RNA Stabilization Solution
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Product RNAlater-ICE Frozen Tissue Transition Solution
RNAlater-ICE Frozen Tissue Transition Solution
Description Prevents RNA degradation during tissue thawing
Sample Types
  • Brain
  • Heart
  • Kidney
  • Spleen
  • Liver
  • Pancreas
  • Lungs
  • Thymus
Product Sizes 25 mL, 10 x 25mL
Protocol RNAlater-ICE Frozen Tissue Transition Solution
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Product Tempus Blood RNA Tubes
Tempus Blood RNA Tubes
Tempus Blood RNA Tubes
LeukoLOCK Total RNA Isolation System
Description Evacuated blood tube designed to lyse and stabilize blood at the point of collection Globin-free RNA from RNAlater–stabilized leukocytes.   Anticoagulated blood is filter-fractionated, keeping only the leukocytes, and stabilized with RNAlater solution for storage
Sample Types
  • Blood
Product Sizes 50 tubes 20 preps
Protocol Tempus Blood RNA Tube and Tempus 12-Port RNA Isolation Kit LeukoLOCK Total RNA Isolation System
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Product The RNA Storage Solution
The RNA Storage Solution
Description Minimizes base hydrolysis by chelating free cations from solution with sodium citrate and by maintaining a low pH
Sample Types
  • Purified RNA
Product Sizes 50 mL, 10 x 1 mL
Protocol The RNA Storage Solution
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Product TRIzol Reagent
RNAlater Stabilization Solution
Description Aqueous, nontoxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA
Sample Types
  • Animal tissues
  • Cells
  • Tissue culture cells
  • E. coli
  • Drosophophila
  • White blood cells
  • Plant tissues
Product Sizes 100 mL, 500 mL, 50 x 1.5 mL, 20 x 5 mL, 1 x 250 mL
Protocol RNAlater Tissue Collection: RNA Stabilization Solution
  Order now
Product RNAlater-ICE Frozen Tissue Transition Solution
RNAlater-ICE Frozen Tissue Transition Solution
Description Prevents RNA degradation during tissue thawing
Sample Types
  • Brain
  • Heart
  • Kidney
  • Spleen
  • Liver
  • Pancreas
  • Lungs
  • Thymus
Product Sizes 25 mL, 10 x 25mL
Protocol RNAlater-ICE Frozen Tissue Transition Solution
  Order now
Product Tempus Blood RNA Tubes
Tempus Blood RNA Tubes
Tempus Blood RNA Tubes
LeukoLOCK Total RNA Isolation System
Description Evacuated blood tube designed to lyse and stabilize blood at the point of collection Globin-free RNA from RNAlater–stabilized leukocytes.   Anticoagulated blood is filter-fractionated, keeping only the leukocytes, and stabilized with RNAlater solution for storage
Sample Types
  • Blood
Product Sizes 50 tubes 20 preps
Protocol Tempus Blood RNA Tube and Tempus 12-Port RNA Isolation Kit LeukoLOCK Total RNA Isolation System
  Order now Order now
Product The RNA Storage Solution
The RNA Storage Solution
Description Minimizes base hydrolysis by chelating free cations from solution with sodium citrate and by maintaining a low pH
Sample Types
  • Purified RNA
Product Sizes 50 mL, 10 x 1 mL
Protocol The RNA Storage Solution
  Order now

Video: How to stabilize RNA in fresh specimens

Learn how to quickly and easily stabilize and protect RNA in fresh specimens. RNAlater Stabilization Solution eliminates the need to immediately process samples, freeze them in liquid nitrogen, or rush them to the freezer.

Revolutionary reagent minimizes the need to immediately freeze samples to preserve RNA

Once saturated in RNAlater solution, tissues can be removed, dissected, or weighed at room temperature, then returned to RNAlater solution for continued storage. Tissues treated with RNAlater solution and archived at –80°C can be subjected to 10 freeze/thaw cycles without affecting the quality of subsequently isolated RNA (Figure 1). Samples stored in RNAlater solution can be shipped on wet ice or even at room temperature if shipped overnight. Figure 2 shows data from a ribonuclease protection assay using RNA from tissue stored in RNAlater solution.

Learn More about RNAlater

RNA Stability of RNAlater-Treated Tissues After Multiple Freeze/Thaw Cycles
Figure 1. RNA Stability of RNAlater-Treated Tissues After Multiple Freeze/Thaw Cycles. Mouse heart and liver were dissected and placed in RNAlater solution. The tissues were frozen on dry ice and thawed at room temperature for the indicated number of cycles. RNA was isolated using RNAWIZ™, electrophoresed on a 1% formaldehyde/agarose gel, ethidium bromide stained, and photographed.
RNA Stability of RNAlater-Treated Tissues After Multiple Freeze/Thaw Cycles

Figure 2. Ribonuclease Protection Assay Using Mouse Tissues Stored in RNAlater Solution. Tissues were stored in RNAlater solution for 1 or 4 weeks at 4°C. RNA was isolated from each tissue and analyzed using the RPA III Kit.


RNA Stabilization Support Materials

TechNotes Articles

  • Make Time Stand Still with RNAlater - TechNotes 10(1): The Ambion RNAlater Tissue Collection: RNA Stabilization Solution that makes it possible for researchers to postpone RNA isolation for days, weeks, or even months after tissue collection without sacrificing RNA integrity.
  • Thaw Frozen Tissues Without Damaging RNA - TechNotes 10(2): RNAlater-ICE solution is a unique RNA-stabilizing solution—simply drop frozen tissues into RNAlater-ICE solution and walk away! The tissues thaw without risk of RNA degradation. Once thawed the tissues can be processed like fresh tissues.

 

Posters

  • RNAlater Solution Preserves Bacterial Gene Expression Profiles for Array Analysis: Here we show that RNAlater solution immediately stabilizes RNA expression profiles in both gram-positive and gram-negative species. RNA was isolated from the bacteria with and without RNAlater solution treatment, and RNA integrity was evaluated on the Agilent 2100 bioanalyzer and by membrane filter–based array analysis.


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