Total Nucleic Acid Extraction / Protein
Research sometimes requires testing of more than just RNA from each sample. For example researchers may want to compare the effects of genetic variations on gene expression, or to evaluate both protein and cognate mRNA levels. To serve this need, some Ambion® purification products are designed to extract both RNA and DNA, or both RNA and protein from samples. Kits with these capabilities allow users to get more out of their samples while employing one kit to streamline the process.
The MELT™ Total Nucleic Acid Isolation Kit provides a method to obtain RNA or DNA from fresh or frozen tissue without physical digestion. For FFPE samples, the RecoverALL™ Total Nucleic Acid Isolation Kit for FFPE sets the standard and allows users to purify both RNA and DNA via a conventional filter-based method, while the new MagMAX™ FFPE Total Nucleic Acid Isolation Kit offers the same RNA and DNA purification from FFPE samples in a magnetic bead format without the need for xylene.
Nucleic Acid & Protein Extraction Kits
The PARIS™ Kit is designed to simultaneously purify total RNA and generate a native lysate suitable for common protein analysis techniques. The related mirVana™ PARIS™ Kit can also be used to obtain both total RNA and native lysate, but it also recovers the small RNA fraction, either along with total RNA or as a fraction enriched for small RNAs.
Total Nucleic Acid Extraction Kits
The MagMAX™ Total Nucleic Acid Isolation Kit purifies both RNA and genomic DNA with a combined mechanical (zirconia-bead beating) and chemical lysis method designed specifically for hard-to-lyse bacteria. Conversely, the MELT™ Total Nucleic Acid Isolation Kit provides a closed-tube, enzymatic method to obtain RNA or DNA from fresh or frozen tissue without physical disruption. For FFPE samples, the RecoverALL™ Total Nucleic Acid Isolation Kit for FFPE is designed to purify both RNA and DNA via a conventional glass fiber filter-based method, while the new MagMAX™ FFPE Total Nucleic Acid Isolation Kit offers xylene-free RNA and DNA purification from FFPE samples using a magnetic bead method.
MELT™ Total Nucleic Acid Isolation System: a new technology for hands-free tissue disruption, RNA preservation and total nucleic acid purification. Nature Methods, 23 Aug 2005.