Thermo Scientific offers a wide portfolio of extraction kits and products for total RNA. Some research studies require the capture of transfer RNA (tRNA), ribosomal RNA (rRNA), micro RNA (miRNA or microRNA), and messenger RNA (mRNA), and our kits are designed to isolate broader RNA—not just mRNA.
Preparation of total RNA isolates all the RNA in a cell including transfer RNA (tRNA), ribosomal RNA (rRNA), messenger RNA (mRNA), and micro RNA (miRNA or microRNA). Starting with high-quality, pure, and intact total RNA is critical to many experiments (e.g., RT-PCR, qRT-PCR, array analysis, northern blots, nuclease protection assays, and RNA-seq).
Choosing the RNA isolation kit that best fits your research workflow starts with what purification method you want to use on your samples. Common methods include organic reagent lysis, magnetic bead separation, and silica column filtration.
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Serving the need to isolate total RNA from a variety of sample types and to different purity levels, there are several Invitrogen total RNA extraction kit options. Different purification methods vary for sample type, as well as the types of downstream experiments in which the total RNA will be used.
Organic solvents for RNA extraction offer proven performance (used in thousands of peer-reviewed studies), a simple method requiring no extra devices, and good value for the laboratory.
TRIzol reagents are monophasic solutions containing phenol and guanidine thiocyanate that offer:
RiboPure kits are designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRIzol reagent with glass-fiber filter purification to yield pure RNA, free of residual proteins and lipids.
Magnetic particles offer solution-phase binding and washing kinetics to facilitate RNA purification and improved sample handling, including automation, ideal for higher throughput processing.
MagMAX mirVana Total RNA Isolation Kit utilizes magnetic beads to purify high yields of total RNA using silica-magnetic particle technology. MagMAX magnetic bead kits offer:
Silica membrane filter columns allow fast and easy RNA extraction resulting in pure total RNA that can be used in most applications. Silica columns employ an easy bind-wash-elute process for the extraction of RNA that is cost-efficient.
Invitrogen PureLink purification columns offer:
|Best for||Gold standard for highly pure, intact RNA||High-quality RNA in less than 30 minutes||High-quality RNA in less than 20 minutes||High-throughput purification of RNA and DNA||No-purification system for qPCR results|
|Product||TRIzol reagents||RiboPure kits||PureLink kits||MagMAX kits||Cells to CT|
|Isolation method||Organic extraction||Organic extraction and silica column||Silica column||Magnetic beads||Chemical lysis and enzymes|
|Prep time||~1 hr|
|20 minutes||~45 minutes||10 minutes|
|Compatible sample types||Most sample types, particularly more difficult to lyse||Tissues, cells, blood, bacteria, yeast||Cells and tissues||Tissues, cells, blood, serum/plasma, urine, plants||Cells|
|Amount of starting material||100 mg of tissue or 107 cells (requires 1 mL reagent)||5–100 mg of tissue or 0.1–20 x 106 cultured cells||Up to 200 mg tissue or 5 x 106 to 1 x 108 cells||Up to 100 mg tissue or 5 x 106 cells||1–100,000 cells|
|Reverse transcription reagents included||✓|
|qPCR master mix included||✓|
|High throughput compatible||✓||✓|
(plate format available)
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
For Research Use Only. Not for use in diagnostic procedures.