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Cells-to-CT KitsExtraction-free RT-PCR results in minutes |
Invitrogen Cells-to-CT kits measure relative gene expression by real-time quantitative PCR (RT-qPCR) without the need for RNA purification prior to amplification. Available in both TaqMan and SYBR Green detection chemistries, Cells-to-CT kits provide a complete workflow for qPCR analysis directly from cultured cells, providing you with excellent performance, reliability, and world-class support.
Perform RT-PCR directly from cell lysates without extraction using the newest product in the Cells-to-CT family. This kit allows you to prepare cells for RT-qPCR analysis in just 5 minutes with a simplified workflow that is suitable for a few samples, or scalable for automated, high‑throughput applications.
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TaqMan Cells-to-CT Express Kit | Cells-to-CT 1-Step TaqMan Kit | Single Cell-to-CT qRT-PCR Kit | TaqMan Fast Advanced Cells-to-CT Kit | |
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Cell working range | 10–100,000 cells | 10–100,000 cells | 1–10 cells | 10–100,000 cells |
1-step or 2-step | 2-step | 1-step | 1-step | 2-step |
SYBR Green Fast Advanced Cells-to-CT Kit | Cells-to-CT 1-Step Power SYBR Green Kit | |
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Cell working range | 10–100,000 cells | 10–100,000 cells |
1-step or 2-step | 2-step | 1-step |
TaqMan Cells-to-CT Express Kit | Cells-to-CT 1-Step TaqMan Kit | Single Cell-to-CT qRT-PCR Kit | TaqMan Fast Advanced Cells-to-CT Kit | |
---|---|---|---|---|
Cell working range | 10–100,000 cells | 10–100,000 cells | 1–10 cells | 10–100,000 cells |
1-step or 2-step | 2-step | 1-step | 1-step | 2-step |
SYBR Green Fast Advanced Cells-to-CT Kit | Cells-to-CT 1-Step Power SYBR Green Kit | |
---|---|---|
Cell working range | 10–100,000 cells | 10–100,000 cells |
1-step or 2-step | 2-step | 1-step |
Cell line | Growth type | Source species | Source tissue |
---|---|---|---|
A549 | adherent | H. sapiens | Lung carcinoma |
BJ | adherent | H. sapiens | Foreskin |
CHO-K1 | adherent | C. griseus (hamster) | Ovary |
COS-7 | adherent | C. aethiops (monkey) | Kidney |
DU-145 | adherent | H. sapiens | Prostate |
HEK-293 | adherent | H. sapiens | Kidney |
HeLa | adherent | H. sapiens | Cervical adenocarcinoma |
HepG2 | adherent | H. sapiens | Liver carcinoma |
Huh-7 | adherent | H. sapiens | Liver |
Jurkat | suspension | H. sapiens | Acute T cell leukemia |
K562 | suspension | H. sapiens | Bone marrow |
ME-180 | adherent | H. sapiens | Cervical epidermoid carcinoma |
NCI-H460 | adherent | H. sapiens | Lung |
Neuro 2A | adherent | M. musculus | Brain |
NIH/3T3 | adherent | M. musculus (mouse) | Embryonic fibroblast |
PC-12 | adherent | R. norvegicus (rat) | Adrenal pheochromocytoma |
PT-K75 | adherent | S. scrofa (pig) | Nasal turbinate mucosa |
Raji | suspension | H. sapiens | B lymphocyte |
SK-N-AS | adherent | H. sapiens | Brain neuroblast |
SK-N-SH | adherent | H. sapiens | Brain fibroblast |
U-2 OS | adherent | H. sapiens | Bone |
U-87 MG | adherent | H. sapiens | Brain glioblastoma |
Note: Due to differences in cell size and composition, the maximum number of cells per lysis reaction may be slightly different for different cell lines. Test for inhibition and minimal sample input by using the TaqMan Cells-to-CT Control Kit.
Leverage our diverse product offerings for real-time, quantitative PCR applications to build a complete experimental workflow solution. Explore our powerful assay design algorithms, optimized master mixes, intuitive data analysis software, and flexible instrumentation to help harness the power of RT-qPCR across a rich and diverse set of applications.
This ASCB presentation shows you how Cells-to-CT kits allow you to measure relative gene expression by qPCR without having to purify RNA prior to amplification.
Learn how to prepare RNA for gene expression analysis from cultured cells, without the need for RNA purification. Fast track to RT-qPCR in only seven minutes.
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
For Research Use Only. Not for use in diagnostic procedures.