Recovery of Total RNA From a Wide Range of Cell Numbers and Sample Types
Recovery of total RNA from a wide range of cell numbers and sample types.(A) RNA was isolated from 25 cells to 2 x 106 cells (K562) in 8 replicate wells using the MagMAX™-96 Total RNA Isolation Kit. Equivalent volumes of the recovered RNA (4% of eluted volume) were used for qRT-PCR (10 µL reactions) targeting human RNA polymerase II mRNA. The percent CV among Ct values for the 8 replicate samples of each cell number input was less than 3%. (B) Analysis on an Agilent® 2100 Bioanalyzer showed highly intact RNA isolated from HeLa cells, mouse kidney, corn leaf, and corn kernels.
For research use only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.