The Invitrogen PureLink RNA Mini Kit provides rapid column based purification of total RNA from a wide range of cell and tissue types.

  • High-quality RNA in less than 20 minutes
  • Up to 1,000 ug of purified RNA from a single extraction
  • No hazardous organic (phenol/chloroform)  extraction lysis
     

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Ready-to-use total RNA

Total RNA isolated using the PureLink RNA Mini Kit can be used in many downstream applications which require high quality total RNA.

  • RT-PCR
  • Real-time PCR
  • cDNA synthesis
  • Nuclease protection assays

PureLink purification technology

Invitrogen PureLink technology combines non-toxic lysis with the speed, purity and ease of use of silica-membrane column RNA purification. The safe and easy RNA purification procedure can be completed in less than 20 minutes, and high quality total RNA can be obtained from mini- to midiprep amounts of starting material with no genomic DNA contamination.

Figure 1. The look of high-quality RNA. RNA was purified from 1x106 HeLa cells TRIzol Reagent, the ChargeSwitch Total RNA Cell Kit, the PureLinkRNA Mini Kit, and the TRIzol Plus Purification System. (A) A260/A280 and A260/A230 and (B) RNA Integrity Numbers (RIN) were assessed using a spectrophotometer and an Agilent Bioanalyzer.

Figure 2. Silica-based purification protocol using Purelink RNA Mini Kit in conjunction with TRIzol Reagent.

Isolate RNA with the PureLink RNA Mini Kit