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PureLink RNA Mini Kit
Fast RNA extraction with PureLink RNA Mini Kit
The Invitrogen PureLink RNA Mini Kit provides rapid column-based purification of total RNA from a broad range of cell and tissue types. Compatible cell and tissue types include mammalian cells, plant cells, yeast cells, bacteria, and blood.
- High-quality RNA in less than 20 minutes
- Up to 1,000 ug of purified RNA from a single extraction
- No hazardous organic (phenol/chloroform) extraction lysis
PureLink RNA Mini Kit to qPCR workflow
RNA purified from tissue culture cells using the PureLink RNA Mini Kit is ready for RT-PCR and subsequent downstream analysis by qPCR using gold-standard Applied Biosystems TaqMan gene expression assays. An example qPCR workflow for gene expression analysis using RNA isolated using the PureLink RNA Mini Kit is shown in Figure 1.
Figure 1. An example of PureLink RNA Mini Kit to qPCR workflow. This workflow begins with RNA isolation from tissue culture using the PureLink RNA Mini Kit. The total RNA obtained from the sample is then ready for RT-PCR with the ProFlex PCR System. Relative amounts of RNA between samples are measured using TaqMan assays via qPCR using the QuantStudio 7 Pro Real-Time PCR System.
How much RNA can be extracted from one million cells using PureLink RNA Mini Kit?
Depending on the cell or sample type and starting amount, the PureLink RNA Mini Kit extracts an average yield of 1.8–350 µg per sample (Table 1). View the list of cells/sample types compatible with the PureLink RNA Mini Kit in Table 2.
Table 1. Average yields of total RNA obtained from various samples using the RNA PureLink Mini Kit
| Sample type | Sample | Starting amount | Average yield (µg) |
|---|---|---|---|
| Animal cells | HeLa cells | 1 x 106 cells | 15–20 |
| 293 cells | 1 x 106 cells | 20–25 | |
Animal tissue | Rat liver | 10 mg 100 mg | 60 300 |
| Rat brain | 10 mg 100 mg | 6 90 | |
| Rat spleen | 10 mg 100 mg | 58 320 | |
| Calf thymus | 10 mg 100 mg | 58 350 | |
| Plant tissue | Arabidopsis | 100 mg | 26 |
| Wheat | 100 mg | 31 | |
| Corn | 100 mg | 36 | |
| Rice | 100 mg | 37 | |
| Alfalfa | 100 mg | 32 | |
| Soybean | 100 mg | 38 | |
| Sugar beet | 100 mg | 31 | |
| 200 mg | 65 | ||
| Yeast cells | S. cerevisiae | 1 x 107 | 1.8 |
| 5 x 108 | 125 |
Table 2. Sample sizes compatible with RNA PureLink Mini Ki
| Source | Amount |
|---|---|
| Animal and plant cells | Up to 5 × 107 cells |
| Animal tissue | Up to 200 mg |
| Bacterial cells | Up to 109 cells |
| Plant tissue | Up to 250 mg |
| Whole blood | Up to 0.2 mL |
| Yeast cells | Up to 5 x 108 cells |
High quality RNA extraction using PureLink RNA Mini Kit
Column kits are available for total RNA, mRNA, miRNA, small RNA, viral RNA, and sequence-specific RNA extractions. Total RNA isolated using the PureLink RNA Mini Kit can be used in many downstream applications, which require high-quality total RNA (Figure 2). These downstream applications include:
- Microarray analysis
- Next generation sequencing (NGS)
- Northern blotting
- Nuclease protection assays
- qPCR
- Real-time PCR
- cDNA library construction and synthesis
Click image to enlarge
Figure 2. The look of high-quality RNA. RNA was purified from 1 x 106 HeLa cells TRIzol Reagent, the ChargeSwitch Total RNA Cell Kit, the PureLink RNA Mini Kit, and the TRIzol Plus Purification System. (A) A260/A280 and A260/A230 and (B) RNA Integrity Numbers (RIN) were assessed using a spectrophotometer and an Agilent Bioanalyzer.
If removal of residual DNA is necessary, the PureLink RNA Mini Kit offers on-column treatment to save time and precious RNA samples, or post-isolation DNase treatment with the PureLink DNase Set.
Silica-based purification protocol using PureLink RNA Mini Kit
PureLink technology combines non-toxic lysis with the speed, purity and ease of use of silica-membrane column RNA purification. The safe and easy RNA purification procedure can be completed in less than 20 minutes, and high-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Click image to enlarge
Figure 3. Silica-based purification protocol using PureLink RNA Mini Kit in conjunction with TRIzol Reagent.
Video: PureLink RNA Mini Kit protocol
Resources
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
- Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
- DNA & RNA Selection Guide—Find the right purification products.
- Videos—View our library of DNA & RNA purification and analysis videos.
- Application Notes—Explore our application notes from scientists sharing data for isolation products
For Research Use Only. Not for use in diagnostic procedures.
Stylesheet for Classic Wide Template adjustments
Stylesheet for Classic Wide Template adjustments
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