RiboPure RNA Isolation Kits combine a robust lysis and denaturing reagent with a glass-fiber filter purification method to provide RNA of high yield and quality. The RNA contains minimal genomic DNA contamination and is ideal for critical downstream applications including RNA amplification for microarray analysis, real-time RT-PCR, and northern blotting.
RiboPure RNA Isolation Kits are suitable for isolation of RNA from a broad range of biological samples, including 5–100 mg fresh, frozen, and RNAlater solution-treated tissues, and 0.1–20 million mammalian cultured cells. RiboPure kits are also available for RNA isolation from blood, yeast, and bacteria. The protocol is fast, simple, reliable, and can be completed in less than 30 minutes.
The RiboPure blood kit is used to isolate high-quality RNA from whole blood. The kit combines two methods of RNA extraction to effectively remove blood components (protein, heme, gDNA, and RNases) that can compromise downstream procedures.
TRIzol (phenol and guanidine thiocyanate reagent monophasic solution) reliably and rapidly lyses cells and simultaneously inactivates nucleases. The use of glass-fiber filters for RNA purification after phase separation simplifies the process and eliminates impurities. The result is exceptionally pure RNA (including microRNA and other small RNAs), free of proteins, organic solvents, and other contaminants.
RiboPure RNA isolation kit for yeast is used to isolate high-quality RNA from yeast without the use of enzymatic pre-treatments that often alter gene expression profiles. Traditional methods of RNA extraction only partially disrupts yeast cells. RiboPure yeast kit includes glass beads to help full disruption of yeast cells, such as Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. Figure 1 shows that the RiboPure yeast kit helps extract high-quality RNA from four different strains of yeast.
Figure 1. Yeast RNA isolated using the RiboPure yeast kit. P. pastoris, S. cerevisiae, C. albicans, and S. pombe total RNA was isolated using the RiboPure yeast kit and treated with DNase. Samples were run on an Agilent bioanalyzer chip. The ratio of 28S:18S rRNA is given for each sample.
The mouse RiboPure blood RNA isolation kit (Cat. No. AM1951) is designed to directly recover total RNA from up to 0.5 mL mouse or rat blood. This kit utilizes collection tubes pre-loaded with RNAlater Solution, a storage reagent that prevents RNA degradation and preserves RNA profiles within samples. RNA isolated by this method has been shown to be useful in a variety of downstream procedures, such as RT-PCR, microarray analysis, and next generation sequencing. This RiboPure kit can be used to isolate total RNA including or depleting small RNAs.
Figure 2. Mouse RNA isolated using the mouse RiboPure blood RNA isolation kit. Total RNA was isolated from 0.5 ml blood from a male A/J strain mouse. The aqueous phase recovered after phenol/chloroform extraction was split into two aliquots: 1) for recovery of total RNA, including miRNA and other small RNAs, and 2) the recovery of total RNA depleted of small RNAs. An aliquot from each preparation separated on Agilent 2100 Bioanalyzer shows the specificity of each protocol. RNA integrity number (RIN) and rRNA ratios—indicators of RNA integrity—are shown on the legend to the right.
Learn more: TechNotes: Improved gene expression profiling with mouse blood
Learn more: TechNotes: RNA from mouse whole blood for expression profiling
Learn more: TechNotes: Quantitate miRNA from a few drops of mouse blood
Mouse RiboPure blood RNA isolation kit protocol
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