LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Interaction Assays
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins,association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand- bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide.
LanthaScreen™ TR-FRET NR Coregulator Interaction Assay Kits contains the reagents necessary to perform an assay to assess the ligand dose dependency for the recruitment of coregulator peptides to nuclear receptor targets.
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. Assays using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.