microarray-analysis-s002905
Sample labeling for expression profiling using microarrays typically involves reverse transcription of RNA or mRNA samples and concurrent or subsequent labeling with a fluorescent dye. SuperScript® III Reverse Transcriptase and Alexa Fluor® dyes deliver superior cDNA labeling for microarrays.

SuperScript® cDNA Labeling Systems

SuperScript® Plus Direct cDNA Labeling Kit

The SuperScript® Plus Direct cDNA Labeling System combines the performance of SuperScript® III RT with the novel Alexa Fluor®-aha-dUTP fluorescently labeled nucleotides, generating highly labeled cDNA that accurately represents your original mRNA sample.

  • Robust cDNA yields without an additional spike
  • Higher signal to background ratios
  • Superior accuracy with improved correlation (R2)

SuperScript® Plus Indirect cDNA Labeling Kit

The SuperScript® Plus Indirect cDNA Labeling System combines the performance of SuperScript® III RT and includes Alexa Fluor® 555 and 647 succinimidyl esters, providing everything needed for sensitive and convenient indirect cDNA labeling in an optimized format.

  • Higher cDNA yields with SuperScript® III
  • Higher signal to background ratios
  • Superior accuracy with improved correlation (R2)

Direct Labeling and Indirect Labeling Systems


Figure 1.  Direct and indirect cDNA labeling protocols.

Direct cDNA labeling—Simple, streamlined protocol for direct incorporation of dye-labeled nucleotides during cDNA synthesis. The Alexa Fluor dyes used in direct labeling have been shown to deliver higher correlation values (R2) than other comparable reagents

Indirect cDNA labeling—Slightly longer protocol which gives high levels of incorporation and equivalent labeling between samples by incorporating amine-modified nucleotides during cDNA synthesis, followed by coupling of the cDNA to an amine-reactive dye.

Improved RNA Amplification

The SuperScript® RNA Amplification System and the SuperScript® Indirect RNA Amplification System, based on the proven linear RNA amplification method developed by Van Gelder et al., enables you to successfully perform expression profiling experiments with as little as 100ng of starting total RNA.

  • Consistent >1000-fold amplification of mRNA
  • Improved gene representation with longer mRNA transcripts
  • Increased gene expression profiling accuracy