QuantiGene Plex Assay Data & Specifications
|Limit of detection||≤ 1,000–2,000 transcripts/assay well|
|Limit of quantitation||≤ 2,000–4,000 transcripts/assay well|
|Linear dynamic range||≥ 3.0 logs|
|Assay CV||≤ 15% intra-assay; ≤ 20% inter-assay|
|Compatible sample types|| Cultured cells, bacteria, whole blood,
PAXgene blood or dried blood spots,
fresh/frozen tissues (animal or plant),
FFPE samples, purified RNA
|Assay format||96-well plate|
Classification of breast cancer cell lines
The QuantiGene™ Plex Assay was used to classify a library of breast cancer cell lines that represents recurrent genetic abnormalities as well as biological variability in primary breast cancer tumors (Figure 1). The data demonstrate strong correlation of the cell lines into basal or luminal subtypes and thus can be utilized to predict response to new drugs for these subtypes of breast cancer.
Analysis of protein and gene expression from the same sample
Human histocytic lymphoma cells, U-937, were treated with 1 μg/mL of LPS. At various time points, cells culture supernatants samples were collected and the corresponding cells were lysed. The supernatants were analyzed for 20 different cytokines using ProcartaPlex Assay. The cell lysates were analyzed for 30 different cytokines using QuantiGene Plex 2.0 Reagent System. The results of protein and gene analysis of two cytokines, IL-8 and IL-1β, are shown below.
For Research Use Only. Not for use in diagnostic procedures.