Assay specifications

Limit of detection ≤ 1,000–2,000 transcripts/assay well
Limit of quantitation ≤ 2,000–4,000 transcripts/assay well
Linear dynamic range ≥ 3.0 logs
Assay CV ≤ 15% intra-assay; ≤ 20% inter-assay
Compatible sample types  Cultured cells, bacteria, whole blood,
 PAXgene blood or dried blood spots, 
 fresh/frozen tissues (animal or plant),
 FFPE samples, purified RNA
  Assay format   96-well plate
  Targets/well   3–80


Classification of breast cancer cell lines

The QuantiGene™ Plex Assay was used to classify a library of breast cancer cell lines that represents recurrent genetic abnormalities as well as biological variability in primary breast cancer tumors (Figure 1). The data demonstrate strong correlation of the cell lines into basal or luminal subtypes and thus can be utilized to predict response to new drugs for these subtypes of breast cancer.

Figure 1: A 12-plex RNA measurement from the cell lines was derived using the QuantiGene Plex Assay and their normalized gene expression levels were plotted using a heat map. Data courtesy of Nicholas Wang, Joe Gray, Lawrence Berkeley National Laboratories, Department of Cancer & DNA Damage Responses. Note: Joe Gray is now with the Oregon Health & Science University, Department of Biomendial Engineering.


Analysis of protein and gene expression from the same sample

Human histocytic lymphoma cells, U-937, were treated with 1 μg/mL of LPS. At various time points, cells culture supernatants samples were collected and the corresponding cells were lysed. The supernatants were analyzed for 20 different cytokines using ProcartaPlex Assay. The cell lysates were analyzed for 30 different cytokines using QuantiGene Plex 2.0 Reagent System. The results of protein and gene analysis of two cytokines, IL-8 and IL-1β, are shown below.

Figure 2: Simultaneous analysis of protein and gene expression from the same sample. Human histocytic lymphoma cells, U-937, were treated with 1 μg/mL of LPS. At various time points, cells culture supernatants samples were collected and the corresponding cells were lysed. The supernatants were analyzed for 20 different cytokines using Procarta Human Immunoassay Kit. The cell lysates were analyzed for 30 different cytokines using QuantiGene Plex Reagent System.