To help researchers in their quest to understand how the genome influences phenotype, we’ve developed a complete set of trusted solutions for every step in the gene editing workflow. Design your CRISPR-Cas9 system or TALEN constructs for precise cleavage, knock-in, and tagging, transfect them efficiently into cells, and validate the genotypic and phenotypic results. Our optimized, validated products, protocols, and tools work together to eliminate the trial-and-error phase and help you get answers faster and with less effort.
Because every lab is unique, we offer a variety of genome editing solutions to meet your needs. Whether you want results fast, seek full control over every design step, or need help engineering cells to your specific requirements, we have solutions that fit.
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We offer tools and solutions for every step in the CRISPR genome editing workflow. Our portfolio of genome editing products is built on 30 years of industry-leading innovation and can grow with your research needs.
Choose from our selection of Cas9 proteins, including our TrueCut Cas9 Protein v2 that delivers consistent high editing efficiency across gene targets and cell types, our high-fidelity Cas9 for minimization off-target effects, or our GMP-manufactured Cas9 for cell therapy applications.
Bypass the trial-and-error phase with step-by-step CRISPR protocols optimized for efficiency, viability, and reproducibility across a range of cell types and gene targets.
Need to get started? Our free Learning Center includes educational articles, videos, webinars, and more covering the latest CRISPR and TALEN methods and best practices.
Genome editing experiments often progress through a basic standard workflow consisting of three design steps, a transfection step, and a validation step. This standard workflow is applicable to gene knockout, tagging, knock-in, and cell line engineering applications. Workflow steps are adaptable to fit each experiment. For example, knockout experiments and functional genomics screening may omit the knock-in step.
Follow the links to learn more about our product offerings for each workflow step. In many cases, the TrueDesign Genome Editor can be used to design and order reagents for the complete editing experiment.
|Design and build||Deliver||Detect and validate|
To see an example of this gene editing workflow in action, view the webinar on using the CRISPR-Cas9 system for CAR T cell knock-in.
When designing your genome edit, you can choose between CRISPR or TALEN technologies.
Although CRISPR-Cas9 gene editing requires a protospacer-adjacent motif (PAM) site in the vicinity of the desired break, this platform is generally viewed as simpler to design and implement. gRNAs can be made at low costs to target nearly any genomic sequence of interest, and expressing multiple gRNAs in cells allows for multiplexing of CRISPR edits.
In contrast, TALEN usage is not dependent on the availability of a PAM site and can be used at any location in the genome. Closer proximity of the TALENs to the target insertion site can also lead to improved homology-directed repair (HDR). Although TALEN may have more specific and flexible capabilities, it is more time consuming and less cost-effective than CRISPR.
|End goal||Permanent gene knockout or knock-in||Permanent gene knockout or knock-in|
|Design restrictions||PAM site close to desired edit locus (NGG for S.pyogenes Cas9)||None|
|More information||Learn more||Learn more|
|Explore tools||Choose Cas9 format||Explore TALEN choices|