GeneArt Engineered Cell Models

Powerful CRISPR models enabling clear results to drive your research forward

The world’s largest collection of engineered cell lines, great for rapid hypothesis testing.

Knockout and knock-in cell lines are the most powerful in vitro models available for understanding gene function. The advent of CRISPR-Cas9 editing technology is providing researchers with the ability to produce nearly any modification they need to dissect biological mechanisms and understand disease processes. However, while CRISPR-Cas9 technology has significantly simplified the process of creating edited cells, isolating and validating individual edited clones can still be a time-consuming process.

Therefore, to accelerate your research using edited cell lines, we have introduced Invitrogen™ GeneArt™ Engineered Cell Models, the world’s largest collection of cell lines engineered using CRISPR-Cas9 technology. The GeneArt Engineered Cell Models collection consists of both pre-engineered knockout and knock-in cell lines.

Search and order     View HAP1 cells in action

Thousands of validated cell lines with defined genome modifications, on the shelf and ready to ship. GeneArt Engineered Cell Models have been engineered using the latest genome editing tools, including CRISPRs and rAAV editing, to create both knock-out and knock-in models.

Advantages of GeneArt Engineered Cell Models:

  • Affordable—academic pricing is comparable to patient-derived cell lines
  • Verified—extensive quality control
  • Comprehensive—the single largest bank of isogenic cell lines
  • Proven—used in hundreds of research labs and published in top scientific journals
  • Available—catalog cell lines can be delivered within one week
  • Controlled—each cell line comes with a matched parental cell line

GeneArt Engineered Cell Models consist of a clonal cell line bearing your mutation of choice, which has been verified by Sanger sequencing. All GeneArt Engineered Cell Models ship with the unedited, isogenic parental cell line, providing the perfectly matched control to enable confidence in your results.

The isogenically matched cell pairs that you receive eliminate the background genetic variation observed with other cell models and provide you with greater confidence that any differences observed between the mutant and the parental cell line are due to your specific mutation of interest.

What QC/validation is performed on the GeneArt Engineered Cell Models?

When appropriate the originating wild type cell line (e.g. SW48) can be single cell diluted to ensure a clonal population exists prior to gene editing. This clone goes through cell line validation (see below) and if successful is taken forward to gene editing.

The resulting clonal mutant cell line goes through the same rigorous cell line validation process.

mRNA expression profile for HAP1

Whole-genome sequencing data, accessible through the NCBI Sequence Read Archive 
under accession numbers SRP044390 (HAP1) and SRP044387 (eHAP A11 and E9).

Scientific advancements happening now can potentially advance our pursuit of life without cancer. We’re committed to accelerating cancer research by pioneering improvements that may impact tumor detection and selection, as well as response to therapy.

Invitrogen™ GeneArt™ Engineered Cell Models include knockout and knock-in gene modifications for colon, breast, lung, connective tissue, pancreas, and skin cancer and leukemia lines. These cell models help researchers better understand the cellular and molecular biology of cancer cells, and how the diseases manifest.

Our engineered cancer cell lines are designed to accurately model cancer-causing mutations found in human samples, while also helping reduce costs associated with drug development steps, including:

  • Target identification
  • Target validation
  • Assay development
  • Drug screening
  • Lead optimization
  • Biomarker-driven clinical trial design

Use the search tool to find cell lines harboring modifications to your genes of interest

Download our complete list

Figure 1. HAP1- a near-haploid human cell line. Genetics is complicated in diploid cells and the reason is that they have two genome copies, one inherited from the mother, and the other inherited from the father. And so, if we introduce a mutation on one of the chromosomes, the phenotype of that mutation is usually masked by the other copy that is still intact. In haploid cells, however, if we introduce a mutation, the phenotype of that mutation will become immediately apparent because there is no second copy buffering the effect.

HAP1 is the workhorse cell line for genome editing experiments

The GeneArt Engineered Cell Models accelerates the cell engineering process by eliminating one of the major challenges to the rapid generation of edited cell lines, the diploid nature of mammalian genomes. Most mammalian cell lines contain two copies of each gene, one on each chromosome, and many of the standard models that are used every day in research, particularly cancer-derived cell lines, exhibit polyploidy and could have 2, 3, 4, or more copies of any given gene.

Multiple gene copies add complexity and time to the genome editing process. When creating a knockout cell line, one needs to ensure that all copies of the gene are knocked out. Similarly, if a homozygous knock-in mutation is desired, then that mutation needs to be introduced to all copies of the gene. This adds time and effort to the creation of an edited cell line since a large number of cells have to be screened in order to identify and validate a clone containing the desired modification in all gene copies.

Haploid cellular models represent an attractive alternative model system for genome editing. Haploid cellular models are easier to generate than traditional mammalian cell lines, as only one allele needs to be targeted. The GeneArt Engineered Cell Models collection utilizes the HAP1 cell line, a near-haploid human cell line, to facilitate rapid gene-editing experiments and allow us to quickly and affordably provides knockout and knock-in cell lines for nearly any gene in the genome.

HAP1 cell models are ideal for understanding the basic mechanisms underlying many biological and disease processes such as cell cycle regulation, DNA damage repair, apoptosis, autophagy, cell-to-cell signaling, viral infectivity, and many more applications. HAP1 models have been extensively characterized; the genome has been sequenced, the complete expression profile is available, and all essential genes have been identified.

Figure 2. Spectral karyotyping in commonly used cell lines including HAP1. Most mammalian cell lines contain two copies of each gene, one on each chromosome, and many of the standard models that are used every day in research, particularly cancer-derived cell lines, exhibit polyploidy and could have 2, 3, 4, or more copies of any given gene.

Read these recent publications to see HAP1 cell lines in action.

Milder forms of muscular dystrophy associated with POMGNT2 mutations Endo Y et al. (2015) Neurol Genet 1(4):e33.

Gene essentiality and synthetic lethality in haploid human cells Blomen VA et al. (2015) Science 350(6264):1092–1096.

DAG1 mutations associated with asymptomatic hyperCKemia and hypoglycosylation of a-dystroglycan Dong M et al. (2015) Neurology 84(3):273–279.

Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid Rimersma M et al. (2015) Hum Mol Genet 24(8):2241–2246.

9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate Baumann AM et al. (2015) Nat Commun 6:7673. 

KPC1-Mediated Ubiquitination and Proteasomal Processing of NF-κB1 p105 to p50 Restricts Tumor Growth  Kravtsova-Ivantsiv Y et al. (2015) Cell 161(2):333–347.

Role of Mouse and Human Autophagy Proteins in IFN-γ–Induced Cell-Autonomous Responses against Toxoplasma gondii  Ohshima J et al. (2014) J Immunol 192(7):3328–3335.

Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line Essletzbichler P et al. (2014) Genome Res (24(12):2059–2065.

Ebola virus entry requires the cholesterol transporter Niemann-Pick C1 Carette JE et al.(2011) Nature 477(7364):340–343.

Lipolysis-stimulated lipoprotein receptor (LSR) is the host receptor for the binary toxin Clostridium difficile transferase (CDT)  Papatheodorou P et al. (2011) Proc Natl Acad Sci U S A 108(39):16422–16427.

HAP1 Expression Reference Tool

The HAP1 Expression Reference tool allows you to quickly check if your gene of interest is expressed in the HAP1 cell line and also to search of engineered cell models by key research area.

Download HAP1 Expression Reference Tool ›

Start searching for your cell line by first selecting your type of organization:

  • Academic organizations include universities, government agencies and nonprofit research institutes.
  • Commercial customers, please identify the size of your organization.
  • To order more than four cell lines, contact us here for a volume discount

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