Nuclear hormone receptors are a family of transcription factors that are activated by lipid-soluble ligands such as steroid hormones, thyroid hormone, vitamin D, and retinoic acid. Nuclear hormone receptors regulate a range of biological processes, including metabolism, development, proliferation, and reproduction. The gene targets within the Invitrogen LentiArray CRISPR Human Nuclear Hormone Receptor Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The gRNA designs for each target were then created using a proprietary gRNA design algorithm developed by the scientists at Thermo Fisher Scientific. The LentiArray CRISPR Human Nuclear Hormone Receptor Library targets 47 genes with up to four gRNAs per gene target (pooled in a single well), for a total of 188 gRNAs. Libraries are delivered as 200 μL of ready-to-use lentiviral particles per gene target at a titer of 1 x 106 TU/mL and are also available as glycerol stocks. Libraries are supplied in 96-well plates.