Transfection is the process by which CRISPR-Cas9 DNA, mRNA and protein systems are introduced into eukaryotic cells. Techniques vary widely and include lipid nanoparticle–mediated transfection, viral delivery, and physical methods such as electroporation. Our Invitrogen Lipofectamine family of reagents paired with the Neon Transfection System offer complete delivery solutions to address your genome editing needs. We have optimized protocols for each product option to help you achieve high cleavage efficiency and the ease of delivery you expect.
Explore the guide below to find the best fit for your gene editing workflow needs.
CRISPR-Cas9 delivery solutions selection guide
Transfection reagents | CRISPR delivery methods | |||
---|---|---|---|---|
CRISPR DNA | CRISPR mRNA | CRISPR protein | LentiViral | |
Lipofectamine CRISPRMAX | ✓ | |||
Lipofectamine 3000 | ✓ | ✓ | ||
NEON Transfection System | ✓ | ✓ | ✓ | ✓ |
Lipofectamine MessengerMAX | ✓ | |||
Lipofectamine Stem (recommended for stem cells) | ✓ | ✓ | ✓ |
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