image of CRISPR Transfection

The CRISPR-Cas9 system greatly simplifies genome editing and has great promise in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants.

Transfection is the process by which CRISPR-Cas9 DNA, mRNA and protein systems are introduced into eukaryotic cells. Techniques vary widely and include lipid nanoparticle–mediated transfection, viral delivery, and physical methods such as electroporation. Our Invitrogen Lipofectamine family of reagents paired with the Neon Transfection System offer complete delivery solutions to address your genome editing needs. We have optimized protocols for each product option to help you achieve high cleavage efficiency and the ease of delivery you expect.

Explore the guide below to find the best fit for your gene editing workflow needs.

CRISPR-Cas9 delivery solutions selection guide

Trasfection reagents CRISPR delivery methods
CRISPR DNA CRISPR mRNA CRISPR protein LentiViral
Lipofectamine CRISPRMAX     ✓  
Lipofectamine 3000 ✓     ✓
NEON Transfection System ✓ ✓ ✓ ✓
Lipofectamine MessengerMAX   ✓    
Lipofectamine Stem
(recommended for stem cells) 		✓ ✓ ✓  

Resources

  • Learning Center
    Access genome editing application resources for more success as you plan and execute your experiments.
  • CRISPR Validated Protocols
    Getting started with CRISPR? Need proven CRISPR validated protocols? Check out our collection of step-by-step validated protocols.
  • FAQs
    Find answers to everyday problems, we have consolidated a list of most commonly asked questions.
  • Webinar Series
    Master the art of CRISPR editing by tuning into our on-demand 3 part webinar series.
  • CRISPR Hands-on Workshop
    Our experienced team has designed a comprehensive four-day workshop to get you up to speed on CRISPR.

Support

For Research Use Only. Not for use in diagnostic procedures.