The CRISPR-Cas9 system greatly simplifies genome editing and has great promise in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants.
Transfection is the process by which CRISPR-Cas9 DNA, mRNA and protein systems are introduced into eukaryotic cells. Techniques vary widely and include lipid nanoparticle–mediated transfection, viral delivery, and physical methods such as electroporation. Our Invitrogen Lipofectamine family of reagents paired with the Neon Transfection System offer complete delivery solutions to address your genome editing needs. We have optimized protocols for each product option to help you achieve high cleavage efficiency and the ease of delivery you expect.
Explore the guide below to find the best fit for your gene editing workflow needs.
CRISPR-Cas9 delivery solutions selection guide
|Trasfection reagents||CRISPR delivery methods|
|CRISPR DNA||CRISPR mRNA||CRISPR protein||LentiViral|
|NEON Transfection System||✓||✓||✓||✓|
(recommended for stem cells)