Artistic rendition of CRISPR Cas9 gene editing with hand removing a section of DNA strand with tweezers

Knock-in genome editing, simplified

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Easily create accurate and more successful knock-in experiments with TrueDesign Genome Editor. Our free design tool has an intuitive point-and-type interface that provides unrivaled flexibility and support for creating genome edits. The simple step-by-step program allows you to:

  • Generate a complete knock-in design in minutes
  • Edit up to 30 bases in any human gene using CRISPR/Cas9 or TALENs to create SNP or amino acids changes.
  • Add a GFP, RFP, or luciferase tag and create adaptor primers for our TrueTag™ Donor DNA Kits  to produce a donor template without cloning
  • Single-click download comprehensive documentation

Read how improved design and delivery of gRNA, Cas9 nuclease, and donor DNA contribute to enhanced CRISPR/Cas9-mediated genome editing

Learn about our latest innovation in gene editing tools. Explore the functionality of the TrueDesign Genome Editor through this detailed application note which details the simple step-by-step program that allows you to:

  • Generate a complete knock-in design in minutes
  • Edit up to 30 bases in any human gene using CRISPR-Cas9 or TALEN technology
  • Design all necessary tools for precise SNP or amino acid changes
  • Design the tools to add a GFP, RFP, or luciferase tag to a target gene without the need for cloning
  • Download or order your comprehensive design and recommended reagents with a single click

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Genome editing workflow with the TrueDesign Genome Editor

Search for your gene

Screenshot of search interface for finding genes and related transcripts to edit within the human genome

Easily navigate the human genome to find the exact transcript and loci you want to modify.

Craft your edit

Screenshot of interface for specifying edits for given nucleic acid and amino acid sequences of a gene on interest

View the DNA and amino acid sequences while editing your gene with point-and-type features.

Complete your design

Screenshot of display and download options for CRISPR-Cas9 gRNA or TALEN designs as well as donor sequences

TrueDesign will generate your specific donor and the associated CRSIPR-Cas9 gRNA or TAL effector nucleases sequences.

Search for your gene

Screenshot of search interface for finding genes and related transcripts to edit within the human genome

Easily navigate the human genome to find the exact transcript and loci you want to modify.

Craft your edit

Screenshot of interface for specifying edits for given nucleic acid and amino acid sequences of a gene on interest

View the DNA and amino acid sequences while editing your gene with point-and-type features.

Complete your design

Screenshot of display and download options for CRISPR-Cas9 gRNA or TALEN designs as well as donor sequences

TrueDesign will generate your specific donor and the associated CRSIPR-Cas9 gRNA or TAL effector nucleases sequences.

Looking for the GeneArt CRISPR Search and Design Tool? This tool can be used to design gRNAs and compatible oligos for our Precision gRNA Synthesis Kit. If designing a variety of knock-in edit choices though, we recommend the TrueDesign Genome Editor.

Release notes for TrueDesign Genome Editor

The major features in this version include the features of Release 1.0 and add the following capabilities:

  1. Add selected oligos, Donor DNA sequences, TrueTag primers, Sequencing/GCD primers, required and recommended products to cart.
  2. Display the progress of CRISPR oligo design in a progress bar percentage.
  3. Display silent mutations on Donor DNA sequences. Silent mutations are introduced into Donor DNA so that they are not disrupted by the Cas9 protein.
  4. Email communication to Services Lab if a user is interested in related TALEN products.

The major features in the initial version include:

  1. Search a gene and list it’s transcripts, and then view the transcript with nucleic acid and amino acid sequences, exonic and intronic annotations, and translated and untranslated annotations.
  2. Make edits on the transcript sequence and design CRISPR-Cas9 and TALEN sequences for accomplishing that edit.
  3. Choose between TrueTag, Insertion, Deletion or SNP change edits.
  4. View the CRISPR-Cas9 and TALEN designs, their scores and recommendations made by the app, select the oligos to view their Donor DNA sequences
  5. Export the CRISPR-Cas9 and TALEN, Donor DNA, and primer sequences, along with the edit information in an Excel sheetfile.

Before getting started, ensure the following:

1).  Is your browser supported? Thermo Fisher Cloud includes the latest data security and display features that require the use of one of the following browsers/versions:

Click on a link above to download and install a compatible browser. Thank you for using Thermo Fisher Cloud.

2). Once you are in a supported browser, you will need to log in one time only to the Thermo Fisher Cloud using your existing username and password. After your first visit to the TrueDesign Genome Editor, you will no longer be required to log in to the Cloud.

  • You will be asked to verify your email address the first time you log in.
  • A verification request email will be sent to the email address on file for your online account.
  • Check your spam folder if you don’t immediately receive this verification request.
  • Future visits to the TrueDesign Genome Editor will not require Thermo Fisher Cloud login.
  • Be sure to bookmark the page for future reference.

Resources

Learning Center
Access genome editing application resources for more success as you plan and execute your experiments.

Simplified CRISPR-Cas9 Protocols
Getting started with CRISPR-Cas9? Need proven genome editing protocols? Check out our collection of step-by-step and cell-line specific recommendations.

FAQs
Find answers to everyday problems, we have consolidated a list of most commonly asked questions.

Webinar Series
Master the art of CRISPR editing by tuning into our on-demand 3 part webinar series.

CRISPR Hands-on Workshop
Our experienced team has designed a comprehensive four-day workshop to get you up to speed on CRISPR.

Support

Complementary Concierge
Make a free appointment to connect with one of our technical experts to get started or troubleshooting your genome editing project.

Support Center
Find tips, troubleshooting help, and resources for your genome editing experiments.

Custom Engineering Services
Contact our engineering services team for custom engineered cell lines, bulk reagents, and validation and testing services.

Contact Technical Support
Find experts who can help you with the technicalities of application and product use.

Commercial Licensing Information
Learn about the commercial opportunities