stylized depiction of an engineered plant

Generating transgenic plants is key to the introduction of new crop traits and carrying out gene studies, both in discovery and applied settings. The development of genetically modified plants often requires a complex design of DNA elements to achieve optimum expression. Based on Xanthomonas effector proteins, GeneArt™ TALs are custom DNA-binding proteins that allow you to accurately target any locus in the genome. TAL effector technology has been shown to be effective in a broad range of organisms, including plants, algae, and fungi. The first of their kind, GeneArt  Precision TALs are ideal for plant research scientists, offering research-use access with licensing for commercial use through the Two Blades Foundation.

Every plant genome locus is within your reach

TALENs derived from Xanthomonas, a genus of plant pathogens. The pathogen specifically recognizes DNA of the host with a protein containing 17–18 repeats of approximately 34 amino acids each. The nucleotide specificity is conferred by amino acids 12 and 13 within each repeat. Each nucleotide in the target DNA is recognized by one repeat, and this 1-to-1 relationship can be used to specifically target any locus in the genome.

GeneArt Precison TALs fit your project and budget

Recent improvements in GeneArt Precison TAL design and manufacturing have allowed us to significantly increase the flexibility and reduce the cost of genome editing with designer GeneArt Precision TALs. Our new pricing should allow all researchers to experience the power of GeneArt® Precison TALs for all-around freedom and unlimited genome targeting.

New pricing!

  • Industrial:  $3,000/pair
  • Academic: $2,000/pair

Ordering GeneArt Precision TALs for Plants

The fastest and easiest way to design, edit, optimize, and order GeneArt Precision TALs is through the GeneArt portal.

Order online ›

The ordering process is simple; just follow these three steps:

  1. Select the functionality (effector domain) of your TAL from Table 1. For details, refer to the ‘TALEN functionality overview’
  2. Select the product name from Table 1.
  3. Then place your order through our online design tool within the GeneArt portal, or you can download (.xls) and complete the TALEN order form and email it to geneartsupport@lifetech.com.

Table 1. Available effector domains

Product DNA-binding
domain		 Effector domain Functionalities   Applications
Native TAL Fok1 Native Fok1 endonuclease Gene targeting
  • Silencing
  • Incorporation of exogenous DNA
Truncated TAL Truncated Fok1 endonuclease Gene targeting
  • Silencing
  • Incorporation of exogenous DNA
Native TAL VP16 Native VP16 activator Activation of transcription
  • Increasing the expression level of endogenous gene isoforms
Native TAL VP64 Native VP64 activator Activation of transcription
  • Increasing the expression level of endogenous gene isoforms
TAL repressor Modified KRAB repressor Epigenetic repression of transcription
  • Heritable knockdown of gene expression
Native TAL MCS Native (MCS) Steric repression & custom design
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice
Truncated TAL MCS Truncated (MCS) Steric repression & custom design
  • Transient knockdown of gene expression
  • Target any locus in the genome with the effector domain of your choice
KRAB = Krüppel associated box; MCS = multiple cloning site; TAL = transcription activator–like (DNA-binding domain).

Resources

Documents

Press release

Web resources

For Research Use Only. Not for use in diagnostic procedures.