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Genome Editing Resource Library

Within the Genome Editing Learning Center, the Resource Library is where you can connect to all our different educational, technical, application, and other learning materials by resource collection. This includes webinars & videos, application notes & poster presentations, validated protocols, and publications.

To browse learning content by genome editing application subtopic, visit the main Genome Editing Learning Center home.

Resource library collections

Genome Editing Videos & Webinars

From general overviews of CRISPR and TALEN-based genome editing techniques and experimental design best practices to specific applications including cell engineering and knock-in/-out strategies, our collection of videos and webinars is designed to help you maximize your success using these technologies.


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TypeNameGenome editing technology
Application NotePowerful loss-of-function screening with LentiArray CRISPR gRNAs: maximize knockout efficiency while maintaining specificityCRISPR-Cas9
Application noteApplication Note: Achieve functional knockout in up to 90% of human primary T cellsCRISPR-Cas9
Application noteDelivery solutions for immune cellsCRISPR-Cas9
Application noteImprove genome editing outcomes in biologically relevant cell modelsCRISPR-Cas9, TALEN
Application noteUsing Sanger sequencing to facilitate CRISPR and TALEN-mediated genome editing workflowsCRISPR-Cas9, TALEN
FAQsCRISPR-based genome editing—Getting Started FAQsCRISPR-Cas9
FAQsCRISPR-based genome editing—Troubleshooting FAQsCRISPR-Cas9
FAQsTALEN-based genome editing—Getting Started FAQsTALEN
FAQsTALEN-based genome editing—Troubleshooting FAQsTALEN
InfographicGenome Editing With Engineered NucleasesCRISPR-Cas9
InfographicThe Evolution of Genome EditingCRISPR-Cas9
ProtocolGeneral CRISPR RNP Transfection GuidelinesCRISPR-Cas9
ProtocolVerification of CRISPR Gene Editing EfficiencyCRISPR-Cas9
ProtocolGuidelines For Clone Isolation and ValidationCRISPR-Cas9
ProtocolCell-Line Specific CRISPR RNP Transfection Conditions Using Lipofectamine CRISPRMAX ReagentCRISPR-Cas9
ProtocolCell-Line Specific CRISPR RNP Electroporation Conditions Using Neon SystemCRISPR-Cas9
ProtocolEmbryo Microinjection With CRISPR-Cas9 in Mice and ZebrafishCRISPR-Cas9
ProtocolCRISPR-Cas9 Genome Editing In Human Induced Pluripotent Stem CellCRISPR-Cas9
Publication (2016)Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNACRISPR-Cas9
Publication (2016)Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAXCRISPR-Cas9
Publication (2015)Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.CRISPR-Cas9
Publication (2015)Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.CRISPR-Cas9
Publication (2015)Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of NucleaseCRISPR-Cas9
Publication (2015)Efficient Intracellular Delivery of Native ProteinsCRISPR-Cas9
Publication (2015)CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.CRISPR-Cas9
Publication (2015)Engineered CRISPR-Cas9 nucleases with altered PAM specificitiesCRISPR-Cas9
Publication (2015)Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancersCRISPR-Cas9
Publication (2015)Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cellsCRISPR-Cas9
Publication (2015)In vivo genome editing using Staphylococcus aureus Cas9CRISPR-Cas9
Publication (2015)Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectorsCRISPR-Cas9
Publication (2015)Multiplex conditional mutagenesis using transgenic expression of Cas9 and sgRNAsCRISPR-Cas9
Publication (2015)Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cellsCRISPR-Cas9
Publication (2015)A Cas9–guide RNA complex preorganized for target DNA recognitionCRISPR-Cas9
Publication (2014)Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleasesCRISPR-Cas9
Publication (2014)Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effectsCRISPR-Cas9
Publication (2014)DNA interrogation by the CRISPR RNA-guided endonuclease Cas9CRISPR-Cas9
Publication (2014)Programmable RNA recognition and cleavage by CRISPR/Cas9CRISPR-Cas9
Publication (2014)Landscape of target: guide homology effects on Cas9-mediated cleavageCRISPR-Cas9
Publication (2014)Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNACRISPR-Cas9
Publication (2014)Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporterCRISPR-Cas9
Publication (2014)Crystal structure of Cas9 in complex with guide RNA and target DNACRISPR-Cas9
Publication (2014)Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo CRISPR-Cas9
Publication (2014)Guide RNA functional modules direct Cas9 activity and orthogonalityCRISPR-Cas9
Publication (2014)Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target CleavageCRISPR-Cas9
Publication (2014)Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding toolCRISPR-Cas9
Publication (2014)GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleasesCRISPR-Cas9
Publication (2014)Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational ActivationCRISPR-Cas9
Publication (2014)Improving CRISPR-Cas nuclease specificity using truncated guide RNAsCRISPR-Cas9
Publication (2014)Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonucleaseCRISPR-Cas9
Publication (2014)High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cellsCRISPR-Cas9
Publication (2014)CRISPR-mediated direct mutation of cancer genes in the mouse liverCRISPR-Cas9
Publication (2014)Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complexCRISPR-Cas9
Publication (2014)Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivationCRISPR-Cas9
Publication (2014)High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairsCRISPR-Cas9
Publication (2014)CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequencesCRISPR-Cas9
Publication (2014)Genome-Scale CRISPR-Cas9 knockout screening in human cellsCRISPR-Cas9
Publication (2014)Genetic screens in human cells using the CRISPR-Cas9 systemCRISPR-Cas9
Publication (2014)Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cellsCRISPR-Cas9
Publication (2014)Dimeric CRISPR RNA-guided FokI nucleases for efficient genome editingCRISPR-Cas9
Publication (2014)CasOT: a genome-wide Cas9/gRNA off-target searching toolCRISPR-Cas9
Publication (2014)Classification and evolution of type II CRISPR-Cas systemsCRISPR-Cas9
Publication (2014)Development and applications of CRISPR-Cas9 for genome engineeringCRISPR-Cas9
Publication (2014)Cut site selection by the two nuclease domains of the Cas9 RNA-guided endonucleaseCRISPR-Cas9
Publication (2014)Synthetic TAL effectors for targeted enhancement of transgene expression in plantsTALEN
Publication (2013)Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexesCRISPR-Cas9
Publication (2013)Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and DeletionsCRISPR-Cas9
Publication (2013)Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegansCRISPR-Cas9
Publication (2013)Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-CasCRISPR-Cas9
Publication (2013)The CRISPR/Cas System mediates efficient genome engineering in Bombyx moriCRISPR-Cas9
Publication (2013)Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in DrosophilaCRISPR-Cas9
Publication (2013)Efficient genome editing in zebrafish using a CRISPR-Cas systemCRISPR-Cas9
Publication (2013)Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafishCRISPR-Cas9
Publication (2013)Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease systemCRISPR-Cas9
Publication (2013)Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas SystemCRISPR-Cas9
Publication (2013)Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryosCRISPR-Cas9
Publication (2013)Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalisCRISPR-Cas9
Publication (2013)Effective gene targeting in rabbits using RNA-guided Cas9 nucleasesCRISPR-Cas9
Publication (2013)Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systemsCRISPR-Cas9
Publication (2013)Heritable gene targeting in the mouse and rat using a CRISPR-Cas systemCRISPR-Cas9
Publication (2013)Generation of gene-modified mice via Cas9/RNA-mediated gene targetingCRISPR-Cas9
Publication (2013)Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA RibonucleoproteinsCRISPR-Cas9
Publication (2013)High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificityCRISPR-Cas9
Publication (2013)Phylogeny of Cas9 determines functional exchange ability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systemsCRISPR-Cas9
Publication (2013)Genotyping with CRISPR-Cas-derived RNA-guided endonucleasesCRISPR-Cas9
Publication (2013)crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilusCRISPR-Cas9
Publication (2013)Programmable DNA cleavage in vitro by Cas9CRISPR-Cas9
Publication (2013)Genome engineering in cerevisiae using CRISPR-Cas systemsCRISPR-Cas9
Publication (2013)RNA-guided editing of bacterial genomes using CRISPR-Cas systemsCRISPR-Cas9
Publication (2013)Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas systemCRISPR-Cas9
Publication (2013)Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9CRISPR-Cas9
Publication (2013)Targeted genome modification of crop plants using a CRISPR-Cas systemCRISPR-Cas9
Publication (2013)Heritable genome editing in C. elegans via a CRISPR-Cas9 systemCRISPR-Cas9
Publication (2013)CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegansCRISPR-Cas9
Publication (2013)Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 SystemCRISPR-Cas9
Publication (2013)Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 NucleaseCRISPR-Cas9
Publication (2013)Multiplex Genome Engineering Using CRISPR/Cas SystemsCRISPR-Cas9
Publication (2013)RNA-programmed genome editing in human cellsCRISPR-Cas9
Publication (2013)Targeted genome engineering in human cells with the Cas9 RNA-guided endonucleaseCRISPR-Cas9
Publication (2013)CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineeringCRISPR-Cas9
Publication (2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cellsCRISPR-Cas9
Publication (2013)DNA targeting specificity of RNA-guided Cas9 nucleasesCRISPR-Cas9
Publication (2013)Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirusCRISPR-Cas9
Publication (2013)CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activityCRISPR-Cas9
Publication (2013)Orthogonal Cas9 proteins for RNA-guided gene regulation and editingCRISPR-Cas9
Publication (2013)Efficient generation of large-scale genome-modified mice using gRNA and Cas9 endonucleaseCRISPR-Cas9
Publication (2013)Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificityCRISPR-Cas9
Publication (2013)Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expressionCRISPR-Cas9
Publication (2013)Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas systemCRISPR-Cas9
Publication (2013)CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotesCRISPR-Cas9
Publication (2013)Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and riceCRISPR-Cas9
Publication (2013)Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNACRISPR-Cas9
Publication (2013)Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidisCRISPR-Cas9
Publication (2013)The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systemsCRISPR-Cas9
Publication (2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. TALEN
Publication (2012)A CRISPR/Cas system mediates bacterial innate immune evasion and virulenceCRISPR-Cas9
Publication (2012)Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteriaCRISPR-Cas9
Publication (2012)RNA-Guided Human Genome Engineering via Cas9CRISPR-Cas9
Publication (2012)A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunityCRISPR-Cas9
Publication (2012)Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joiningCRISPR-Cas9
Publication (2011)The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coliCRISPR-Cas9
Publication (2011)CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIICRISPR-Cas9
Publication (2011)A TALE nuclease architecture for efficient genome editing TALEN
Publication (2011)Assembly of custom TALE-type DNA binding domains by modular cloningTALEN
Publication (2011)Genetic engineering of human pluripotent cells using TALE nucleasesTALEN
Publication (2011)Transcriptional Activators of Human Genes with Programmable DNA-SpecificityTALEN
Publication (2011)Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targetingTALEN
Publication (2011)Efficient construction of sequence-specific TAL effectors for modulating mammalian transcriptionTALEN
Publication (2009)Short motif sequences determine the targets of prokaryotic CRISPR defence systemCRISPR-Cas9
Publication (2009)Breaking the code of DNA binding specificity of TAL-type III effectorsTALEN
Publication (2009)A simple cipher governs DNA recognition by TAL effectorsTALEN
Publication (2007)CRISPR provides acquired resistance against viruses in prokaryotesCRISPR-Cas9
Publication (2005)Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal originCRISPR-Cas9
Resource guideCRISPR-Cas9 Protein for Genome Editing—Getting Started GuideCRISPR-Cas9
Resource guideGenome Editing Resource GuideCRISPR-Cas9, TALEN
VideoGenome editing video collectionCRISPR-Cas9
WebinarGenome editing webinarsCRISPR-Cas9, TALEN