A fast, simple, and precise method to perform gene edits
The transformative CRISPR-Cas9 technology is revolutionizing the field of genome editing. Able to achieve highly flexible and precise targeting, the CRISPR-Cas9 system can be modified and redirected to become a powerful tool for genome editing in broad applications.
From designing, synthesizing, and validating your CRISPR process, we offer end-to-end services to support every step in genome editing. Our dedicated R&D scientists and technical support specialists are ready to partner with you and your colleagues to accelerate your research program.
Available CRISPR-Cas9 engineering services
Choose from a number of services to customize your genome engineering project. Also see our custom CRISPR cell line engineering services.
Table 1. Recommended gene editing technology formats based on applications and outcomes
|Format||CRISPR-Cas9 protein (RNP)||CRISPR-Cas9 mRNA||TAL mRNA||CRISPR-Cas9 plasmids|
|Product||IVT gRNA + Cas9 nuclease||IVT gRNA + Cas9 mRNA||TAL mRNA||AiO CRISPR-Cas9 (OFP/CD4) plasmids|
|Cell type||Most cell types||Most adherent cell lines||Most cell types||Easy to transfect cells|
|*Note: improve % HDR when cut site is less than 10 bp from SNP|
We’ve developed robust algorithms that allow us to generate the most optimal CRISPR-Cas9 gRNA designs, taking into consideration potential off-target effects.
Based on our experience, we recommend that you design and test at least 3 gRNAs and choose the design that provides you with the highest on-target efficiency and minimal off-target effects. We offer a validation service where we design and test at least 3 designs and then provide you with the best-performing gRNAs.
Additional CRISPR-Cas9 validation services
- Validate CRISPR-Cas9 gRNA design efficacy via genome cleavage detection (GCD) assay
- Validate CRISPR-Cas9 gRNA design efficacy via on-target next-generation sequencing (NGS) analysis
- Verify lack of off-target effects by NGS using Ion AmpliSeq™ technology
Need assistance with CRISPR gRNA design?
Our CRISPR Search & Design tool allows you to search our database of >600,000 predesigned CRISPR gRNAs in human and mouse genes or analyze your sequence of interest for de novo gRNA designs using our proprietary algorithms. Up to 25 gRNA sequences per gene are provided with recommendations based on potential off-target effects for each CRISPR sequence.
Discover how our off-the-shelf product portfolio and outsourced services can meet your needs. Contact our dedicated technical support team with any questions, or get started on your services project today:
For Research Use Only. Not for use in diagnostic procedures.