Using SYBR® Green and
TaqMan® Chemistry
for real-time PCR

Both SYBR® Green and TaqMan® chemistry can be used with real-time PCR analysis to measure gene expression, but they provide different levels of specificity and sensitivity. Watch the videos below and learn more about SYBR® Green and TaqMan® chemistry, and their use in real-time PCR analysis.

In addition, learn how to better understand the results achieved using SYBR® Green chemistry by watching the video below about multiple peaks in the SYBR® Green melt curve. It helps explain how multiple melt curves can impact the interpretation of your real-time PCR results.


How TaqMan chemistry works
Finding Multiple Melt Curves
Multiple peaks in the SYBR melt curve
Scientist in lab

Watch the rap battle and learn how to vote for your favorite chemistry
Rap Battle: Taqman vs SYBR

Compare SYBR Green chemistry and TaqMan chemistry for real-time PCR.

Watch video ›

Order SYBR Green master mixes
Order TaqMan assays