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Oligos Tools & Utilities Hub
Oligos Tools & Utilities Hub
Design, create, and assemble the tools you need for exploring genomes
We offer a broad range of development and analytical tools, collections of assays, and analytical and management software that allow researchers to fully interrogate individual genes, complex genetic pathways, and whole genomes: Read moreRead less
- Employ calculators to analyze oligos including concentrations, sequence-specific properties, melting temperatures, and other parameters
- Design basic or highly modified oligos and create primers for PCR, sequencing, or cloning
- Generate specially modified or dye-labeled probes and build gene fragments or whole libraries to assess complex pathways
- Browse collections of preconfigured assays and gene editing and silencing tools to assist in genome-based studies
General oligo tools
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CALCULATEOligo calculators
Calculate concentrations, estimate percent full-length product and yield by length, synthesis scale, and purification method
DESIGN
Invitrogen OligoPerfect designer
Invitrogen OligoPerfect designer
Design primers for PCR, CE sequencing, restriction enzyme cloning, TOPO and Gateway cloning
Sign-in required 
Amplification
Most Popular
CALCULATETm calculator
Calculate the melting temperature of primers and estimates an appropriate annealing temperature when using different DNA polymerases
SEARCH
TaqMan real-time PCR assays
TaqMan real-time PCR assays
Search over 10 million predesigned Applied Biosystems TaqMan assays and TaqMan Arrays for gene expression, SNP genotyping, microarray analysis, copy number, and mutation detection applications
DESIGN
Primer express
Primer express
Design custom primers and probes for your real-time PCR experiment
Gene expression
Most Popular
SEARCHTaqMan gene expression assays and arrays
Search over 2 million predesigned assays covering more than 30 species in a single-tube, 96-well plate, 384-well microfluidic card, and Applied Biosystems OpenArray formats
DESIGN
Custom TaqMan assay design tool (CADT) for gene expression
Custom TaqMan assay design tool (CADT) for gene expression
Design your own Custom or Custom Plus TaqMan Gene Expression Assays
DESIGN
Custom TaqMan small RNA tool
Custom TaqMan small RNA tool
Design your own custom assays for small RNA targets
Genotyping
Most Popular
SEARCHTaqMan SNP genotyping assays
Search over 7 million SNP assays for validation and screening studies of disease association and population stratification applications
DESIGN
DNA sequence prep tool
DNA sequence prep tool
Prepare gDNA targets for submission into the Custom TaqMan Assay Design Tool (CADT) for Custom TaqMan SNP Genotyping Assay design with this automated tool
DESIGN
Custom TaqMan assay design tool (CADT) for genotyping
Custom TaqMan assay design tool (CADT) for genotyping
Design your own Custom SNP Genotyping Assays
DESIGN
GeneAssist copy number assay workflow builder
GeneAssist copy number assay workflow builder
Design your own Custom and Custom Plus Copy Number Assays
Sequencing
Most Popular
SEARCHIon AmpliSeq NGS panels
Browse panels of oligonucleotide primer pairs designed for SNP, CNV, fusion, and indel detection
SEARCH
Primer designer tool for Sanger sequencing
Primer designer tool for Sanger sequencing
Search our database of approximately 650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome
Cloning
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ANALYZEVectorNTI
Manage, view, analyze, transform, share, and publish diverse types of molecular biology data with this integrated suite of sequence analysis and design tools
Gene synthesis
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DESIGNGeneArt gene synthesis
For chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence
DESIGN
GeneArt strings DNA fragments and libraries
GeneArt strings DNA fragments and libraries
Build custom synthetic gene fragments (up to 3 kb) and libraries (up to 2 kb) with full IUPAC options, ready for cloning
Genome editing
Most Popular
DESIGNGeneArt tal effector search and design tool
Design custom TAL effectors or search over 600,000 predesigned TALENs targeting human and mouse genes
SEARCH
GeneArt CRISPR search and design tool
GeneArt CRISPR search and design tool
Search over 600,000 predesigned CRISPR guide RNAs (gRNAs) targeting human and mouse genes or analyze your sequence of interest for de novo gRNA designs using our proprietary algorithms
Functional genomics
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SEARCHsiRNA selection tool
Search over 200,000 Ambion siRNAs available to effect gene specific silencing
SEARCH
GeneAssist custom siRNA builder
GeneAssist custom siRNA builder
Select from ordering tools for custom designed, synthesized, and modified siRNAs and assays
SEARCH
miRNA mimics/inhibitors
miRNA mimics/inhibitors
Search over 2000 Invitrogen miRNA mimics and inhibitors
DESIGN
Basic RNA Oligo ordering
Basic RNA Oligo ordering
Order custom Stealth RNAi or RNA oligos
Application notes, handbooks and sourcebooks, webinars, white papers, how-to videos, case studies, scientific posters, product selection guides, and more.
Find resources and tips for getting started, and troubleshooting help all in one location.
For Research Use Only. Not for use in diagnostic procedures.