Thermo Fisher Scientific offers non-standard, structural phosphoramidites for synthesis of modified oligonucleotides. Oligos with these modifications can then be used as primers, probes, or in structural studies.
Stability and melting point of oligo duplexes can be altered by incorporation of N4-Ethyl dC, 5-Me dC, dI, or dU into the sequence, while mutagenic effects from methylation of an exocyclic amine can be studied by the incorporation of N6-Me-dA. Similar studies may be performed utilizing 8-oxo-dG, which mimics an oxidized guanine.
Structural phosphoramidites can be custom manufactured with base or sugar (2′, 3′ or 5′ positions) modifications.
Biotin-TEG can be placed throughout the oligo (3', 5', or internal) while also having a spacer arm, thereby minimizing steric hindrance between the biotin moiety and the oligo. 5'-Biotin amidite can only be synthesized to the 5′ end of the oligonucleotide.
Structural phosphoramidites by synthesizer compatibility
| Synthesizer | ABI/AKTA | Expedite | Mermade | Bulk |
|---|---|---|---|---|
| Specialty | 5me-dC | - | Contact us | Contact us |
| dI | dI | |||
| dU | dU | |||
| 8-oxo-dG | - |
Modified Phosphoramidites - 5-Me-dC, dI, and dU
Thermo Scientific modified nucleoside 5-Me dC, dI, and dU phosphoramidites are used in specialty applications requiring high quality and performance. The unique deoxy-Inosine (dI) phosphoramidite structure facilitates pairing with all four bases, and is often utilized as a degenerate base for oligo probes/primers.
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5-Me-dC CEP
dI CEP
dU CEP
| 5-Me-dC CEP | dI CEP | dU CEP | |
|---|---|---|---|
| Cat. No. | 27-1748 | 27-1744 | 27-1738 |
| Phosphoramidite Description | 5′-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-5-Methyl-2′-Deoxycytidine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-O-(4,4'-Dimethoxytrityl)-2′-Deoxyinosine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-O-(4,4'-Dimethoxytrityl)-2′-Deoxyuridine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Appearance | Color intensity not to exceed Pantone #P1-4U or #P4-2U | NA | |
| Formula | C47H54N5O8P | C40H47N6O7P | C39H47N4O8P |
| Molecular weight | 847.95 g/mol | 754.8 g/mol | 730.8 g/mol |
| CAS # | 105931-57-5 | 141684-35-7 | 144385-59-1 |
| Solution Clarity | Free from undissolved particulate at 0.2M in ACN | NA | |
| HPLC purity | ≥ 98% | ≥ 98.0% | ≥ 98.0% |
| 31P NMR purity | ≥ 98% | ≥ 98% | ≥ 98.0% |
| Water content | ≤ 0.3% (by KF method) | ||
| Shipping/Storage condition | Ambient/–20 °C | ||
| Material Origin | non-animal source (no TSE/BSE risk) | ||
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Modified Phosphoramidites - N6-Me-dA, N4-Ethyl-dC, and 8-oxo-dG
Thermo Scientific modified nucleoside N6-Me-dA, N4-Ethyl-dC, and 8-oxo-dG phosphoramidites are used in specialty applications requiring high quality and performance. Modified phosphoramidites are used to synthesize oligonucleotides with base modifications that can alter the stability and melting point of duplexes.
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N4-Ethyl-dC CEP
N6-Methyl-dA CEP
8-oxo-dG CEP
| N4-Ethyl-dC CEP | N6-Methyl-dA CEP | 8-oxo-dG CEP | |
|---|---|---|---|
| Cat. No. | 27-1743 | 27-1746 | 27-1424 |
| Amidite Description | 5′-O-(4,4'-Dimethoxytrityl)-N4-Ethyl-2′-Deoxycytidine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-O-(4,4'-Dimethoxytrityl)‑N6-Methyl-2′-Deoxyadenosine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-O-(4,4'-Dimethoxytrityl)‑N2-isobutyryl-8-oxo-deoxyguanosine-3′-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula | C41H52N5O7P | C41H50N7O6P | C44H54N7O9P |
| Molecular weight | 757.85 g/mol | 767.9 g/mol | 855.9 g/mol |
| CAS # | 195535-80-9 | 105931-58-6 | 143060-53-1 |
| Solution Clarity | Free from undissolved particulate at 0.1M in ACN |
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| HPLC purity | ≥ 98% | ≥ 98.0% |
|
| 31P NMR purity | ≥ 98% | ≥ 98.0% | |
| Water content | NA | ≤ 0.3% | |
| Shipping/Storage condition | Ambient/–20 °C | ||
| Material Origin | non-animal source (no TSE/BSE risk) | ||
| Request a quote | Request a quote | Order now | |
| 5′-O-(4,4'-Dimethoxytrityl) |
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Biotin (Indirect-labeled) Phosphoramidites
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5-Biotin CEP
Biotin TEG CEP
| 5′-Biotin CEP | Biotin TEG CEP | ||
|---|---|---|---|
| Cat. No. | 27-1793 | 27-1773 | |
| Phosphoramidite Description | [1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminohexyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite | 1-Dimethoxytrityloxy-3-O-(N-biotinyl-3-aminopropyl)-triethyleneglycol-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | |
| Formula | C46H64N5O6PS | C52H76N6O11PS | |
| Molecular weight | 846.08 g/mol | 1010.24 g/mol | |
| Appearance | White solid | ||
| HPLC purity | ≥ 95.0% | ≥ 90% | |
| 31P NMR purity | ≥95.0% | N/A | |
| Solution Clarity | 0.1M solution in ACN is clear | ||
| Shipping / Storage condition | Ambient / -20 °C | ||
| Request a quote | |||
Process and supply chain control
- A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
- Purity of each phosphoramidite lot is assessed by HPLC and 31P NMR analyses
- Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide and result in the accumulation of by-products
- Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility
Flexible packaging
- Common oligo synthesizer pack sizes are available for immediate delivery
- Custom and bulk packaging are also available. Contact us to learn more.
Custom services
- Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
- Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications
Not finding what you are looking for? We also offer DNA phosphoramidites, RNA phosphoramidites, dye-labeled phosphoramidites, 2′-modifications, linker and spacer phosphoramidites, and succinates and CPG support synthesis supports. Contact us to learn more!
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For Research Use Only. Not for use in diagnostic procedures.
