Digital PCR made simple
 

Applied Biosystems is the industry-leading brand choice for genetic analysis, with robust real-time PCR assays powered by our unique design algorithms and years of experience. Our easy-to-use predesigned assay technology is now making digital PCR (dPCR) simple with Applied Biosystems Absolute Q Digital PCR assays.


Benefits of predesigned Absolute Q Digital PCR assays

5_-nuclease-assay

Predesigned Absolute Q dPCR assays consist of a forward primer, one or more target-specific probes, and a reverse primer pre-mixed together at specified concentrations. They require no further design, optimization, or verification. Just add your sample and reagent then run your experiment.

  • Simple—preformulated and verified assay in a single tube, enabling you to immediately start your research
  • Fast—minimal hands-on time. Results in 90 minutes when used with the QuantStudio Absolute Q Digital PCR System
  • Dependable—analyze your data with confidence using verified assays backed by a performance guarantee*


Proven chemistry—proven performance
 

Absolute Q dPCR assays are based on the same gold-standard TaqMan chemistry used in real-time PCR and cited in more than 200K research publications. The assays feature a proprietary probe with a minor groove binder (MGB) moiety at the 3’ end that increases the melting temperature (Tm) of the probe. This means MGB probes can be significantly shorter than traditional approaches, providing better sequence discrimination and flexibility to accommodate more targets in a single reaction.

TaqMan-Probe-illustration_stacked-v2

We stand behind every predesigned Absolute Q Assay you buy from us

We guarantee the performance of all of our predesigned Absolute Q assays for dPCR experiments. Our application‑specific portfolio of assays enables you to obtain the highest quality and performance available. These assays are designed and verified using up-to-date annotations and gold-standard Applied Biosystems TaqMan chemistry.

If an Absolute Q Digital PCR Assay does not perform according to conformance documentation, we will replace it at no cost or credit your account.*

Learn more about the Absolute Q dPCR Assay guarantee ›


Embrace simplicity—explore application-specific assays

Liquid biopsy dPCR image

Mutation detection and monitoring

Absolute Q Liquid Biopsy Digital PCR assays enable reproducible, specific detection of known somatic mutations. Each predesigned assay has been validated using controls to detect and quantify cancer-related mutations at 0.1% allele frequency.

Explore Absolute Q Liquid Biopsy dPCR assays

Absolute Q Viral Titer dPCR image

Cell & gene therapy development solutions

Absolute Q Viral Titer Digital PCR assays enable easy, accurate, absolute quantification of viral vector genomes to measure concentration and evaluate quality for biopharma and gene therapy development. These assays can be run individually or multiplexed using a custom assay for your target gene of interest.

Explore Absolute Q Viral Titer dPCR assays

Absolute Q Viral Titer dPCR image

SARS-CoV-2 wastewater surveillance solutions

Simplify SARS-CoV-2 wastewater surveillance with the Absolute Q SARS‑CoV‑2 Wastewater Surveillance Kit. Help save time while maintaining confidence in yoour results with our wet lab–verified assay targeting SARS‑CoV‑2 N1 and N2 gene regions and a pepper mild mottlevirus normalization target.

Explore Absolute Q Viral Titer dPCR assays


Custom assay design for ultimate control
 

If you can’t find a predesigned assay that fits your needs, or you want to design an assay with your own primer/probe sequence, we can help. Contact us and our assay experts can help design a digital PCR assay formulated for success based on your target sequence of interest or utilizing the primer/probe sequence of your own design.

Learn more

 *Terms and conditions apply. For complete details of the guarantee, go to thermofisher.com/absoluteqassayguarantee

For Research Use Only. Not for use in diagnostic procedures.

Style Sheet for Global Design System