This resource center is a centralized location for the presentation of educational resources across a wide array of digital PCR application areas. Learn more about the most common applications of digital PCR and discover featured blog posts, application notes, videos, and webinars.

 

Applications of digital PCR

 

Oncology Hematological

Cancer research

Improve detection of mutations and quantification of low allele fractions in challenging samples such as liquid biopsy with high specificity, precision and accuracy of dPCR. 

Cell Therapy

Cell and gene therapy research

Measure common viral titer backbones, custom genes of interest, or the presence of residual DNA without the use of a standard curve for faster, simpler, more precise quantification.

Water Faucet

Environmental surveillance

Accurately measure viral load and pathogens or other biomarkers in environmental samples such as wastewater by absolute quantification with improved performance compared to qPCR in the face of common PCR inhibitors.

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Absolute quantification

Eliminate the reliance on a standard curve and improve precision of quantification and robust measurement of target molecules such as next-generation sequencing libraries or to validate sequencing results.

Copy Number Variation

Copy number variation

Accurate and precise quantification of gene copy number variation (CNV) in a single digital PCR well with exceptional discrimination of CNVs with high copy number.

Gene Expression

Gene expression

Fast, precise, and reproducible quantification of gene expression, including rare mRNAs or miRNAs, without a standard curve.

Digital PCR

Multiplexing

Combine multiple nucleic acid targets into a single digital PCR reaction using optical multiplexing of fluorescent probes to improve the number of datapoints per reaction or use multiple probes to identify and quantify unique molecular signatures such as gene editing events.

ResearchEdition

Analysis of rare or limited samples

Quantification of rare or limited samples by dPCR is more precise and reproducible with the technology’s single molecule level limit of detection and standard curve free absolute quantification.

Featured digital PCR resources

Read more about digital PCR technology or download our free infographic to learn the differences between and benefits of digital PCR and qPCR technologies.

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Digital PCR webinars and on-demand events

 

On-Demand Webinar: A digital PCR-based protocol to detect and quantify RNA editing events at hotspots

Abstract: In this webinar, invited speaker Dr. Rémi Buisson of the University of California Irvine describes their development of a simple protocol for accurate quantification of RNA-editing activity using digital PCR. This assay allows rapid absolute quantification of RNA editing events caused by APOBEC3A in cell lines or patient samples.

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On-Demand Webinar: Applications of multiplex digital PCR: Monitoring wastewater for SARS-CoV-2 and measuring bacterial gene expression

In this webinar, invited speaker Dr. Joan L. Slonczewski of Kenyon College describes their ongoing research, which includes the use of the QuantStudio Absolute Q Digital PCR System to monitor and quantify SARS-CoV-2 viral RNA from the wastewater influent of a village community including college residence halls.

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Digital PCR videos

 

In the featured videos below, see the fast, simple, and robust QuantStudio Absolute Q Digital PCR System in action.

Learn why the QuantStudio Absolute Q dPCR System is the number one solution for simple, fast, and reliable digital PCR.

Getting started with the QuantStudio Absolute Q dPCR System is simple and takes under five minutes to prepare a MAP16 digital PCR plate for a run.

 

Customer testimonials

 

Learn why our customers chose powerfully simple digital PCR to meet their unique research needs.

Cancer research, circadian rhythms, RNA base editors

University of California Irvine, Chao Comprehensive Cancer Center

Learn more about how to use digital PCR for cancer research applications ›

Wastewater and environmental surveillance

University of California Santa Cruz

Learn more about digital PCR for environmental surveillance ›

Gene editing

University of Wisconsin Madison

 

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Digital PCR blog posts and publications

 

Blog posts

What is digital PCR?

Learn the basics of digital PCR, how a reaction is digitized, how digital PCR data is collected.
Key topics: digital PCR basics, digital PCR statistics, digital PCR workflow

 

Digital PCR documents

 

Style Sheet for Global Design System