PCR Essentials—Sample Prep
|Life Technologies nucleic acid purification products are optimized to provide maximum yield, purity, and integrity from virtually any sample type. Explore our Ambion® and Invitrogen™ technology choices for efficient nucleic acid purification and quantitation for endpoint PCR, real-time PCR, or other applications.|
Working with RNA?
RT-qPCR is a multi-step protocol that requires both high-quality RNA as input material, and optimal conversion of this RNA to a DNA copy for accurate real-time PCR detection (qPCR).
Optimization is critical at each step. Click on the steps below to see more information and find the associated products.
Working with DNA?
We offer products for the rapid, effective isolation and purification of genomic and plasmid DNA from a variety of samples (including tissues), as well as products that facilitate everything from isolation to PCR amplification in the same tube.
Reverse transcription and cDNA synthesis
Your reverse transcriptase (RT) choice matters
A critical component to obtaining high yields of quality, full-length cDNA with full gene representation is the enzyme choice for cDNA synthesis. Get to know the importance of RNase H, and why not all RTs are created equal.
Learn reverse transcription basics >
Attend a webinar on cDNA synthesis and learn why suboptimal reverse transcription reactions can lead to low yields of cDNA or truncated cDNA. Register for the webinar now >
Which reverse transcriptase is right for you?
We offer a wide selection of high-quality reverse transcriptases to meet your research needs. A variety of cDNA synthesis kits and stand-alone enzymes are available for the flexibility needed to perform your experiments.
|Watch this how-to video on SuperScript® VILO™ cDNA Synthesis Kit and learn how it can improve the dynamic range of your real-time qPCR assays.|