SYBR® Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. Use our selection guide to find the product that's right for you.
SYBR® Master Mix and SuperScript® RT Product Citations
For more than 30 years, SYBR® real-time reagents and SuperScript® reverse transcriptases have been used by researchers around the world. Referenced in over 3,000 peer-reviewed publications, both products have been successfully used in a wide range of applications, including gene expression analysis.
Citations for the use of both SYBR® and SuperScript® reagents
|Mutations in the GW-repeat protein SUO reveal a developmental function for microRNA-mediated translational repression in Arabidopsis||Yang L, Wu G, Poethig R||Proc Natl Acad Sci U S A (2012) 109(1): 315–320||Quantitative RT-PCR (qRT-PCR) revealed that suo-2 and suo-3 significantly reduce the abundance of the SUO transcript. qRT-PCR was performed using SuperScript® II reverse transcriptase (Invitrogen™) and Power SYBR® Green PCR master mix (Applied Biosystems®).|
|Nfatc1 orchestrates aging in hair follicle stem cells||Keys B, Segal J, Heller E, Lien W-H, Chang C-Y, Guo X, Oristian D, Zheng D, Fuchs E||Proc Natl Acad Sci U S A (2013) 110(51): E4950–E4959||qRT-PCR was used to measure the response of NFATc1 targets 48 hours post-depilation. RNA was reverse-transcribed using the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen™). Power SYBR® Green PCR Master Mix (Applied Biosystems®) was used for qPCR.|
|General anesthesia alters time perception by phase shifting the circadian clock||Cheeseman J, Winnebeck E, Millar C, Kirkland L, Sleigh J, Goodwin M, Pawley M, Bloch G, Lehmann K, Menzel R, Warman G||Proc Natl Acad Sci U S A (2012) 109(18): 7061–7066||Clock gene mRNA levels were quantified in individual bee brains via RT-qPCR. cDNA synthesis was performed with 200 ng of RNA and primed with random hexamers using the SuperScript® III first-strand synthesis system (Invitrogen™). Power SYBR® Green PCR Master Mix (Applied Biosystems®) was used for qPCR.|
|Establishment of smooth muscle and cartilage juxtaposition in the developing mouse upper airways||Hines E, Jones M-K, Verheyden J, Harvey J, Sun X||Proc Natl Acad Sci U S A (2013) 110(48): 19444–19449||Muc5ac, Scgb1a1 transcript levels were determined by qRT-PCR. The reverse transcription reaction was done using the SuperScript® III First Strand Kit (Invitrogen™). Power SYBR® Green PCR Master Mix (Applied Biosystems®) was used for qPCR.|
|The estrogen receptor-‘alpha’-induced microRNA signature regulates itself and its transcriptional response||Castellano L, Giamas G, Jacob J, Coombes C, Lucchesi W, Thiruchelvam P, Barton G, Jian L, Wait R, Waxman J, Hannon G, Stebbing J||Proc Natl Acad Sci U S A (2009) 106(37): 15732–15737||Expression levels of the ERa-regulated gene GREB1 were assessed by RT-qPCR. For RT-qPCR assays, cDNA was synthesized using the SuperScript® III First-Strand Synthesis System (Invitrogen™); Power SYBR® Green PCR Master Mix (Applied Biosystems®) was used for qPCR.|
|RIG-I-like receptor LGP2 protects tumor cells from ionizing radiation||Widau R, Parekh A, Ranck M, Golden D, Kumar K, Sood R, Pitroda S, Liao Z, Huang X, Darga T, Xu D, Huang L, Andrade J, Roizman B, Weichselbaum R, Khodarev N||Proc Natl Acad Sci U S A (2014) 111(4): E484–E491||IFN-β expression relative to GAPDH was determined by real-time PCR. Total RNA was reverse-transcribed using SuperScript® III reverse transcriptase (Invitrogen™), and Fast SYBR® Green Master Mix (Applied Biosystems®) was used for real-time PCR.|
|FZD1 regulates cumulus expansion genes and is required for normal female fertility in mice||Lapointe, E, Boyer A, Rico C, Paquet M, Franco H, Gossen J, DeMayo F, Richards J, Boerboom D||Biol Reprod (2012) 87(5): 104||The quantitative RT-PCR analyses of known Wnt4-regulated genes and Fzd1-regulated genes were performed using the SuperScript ®VILO™ cDNA Synthesis Kit (Invitrogen™) and Power SYBR® Green PCR Master Mix (Applied Biosystems®).|
Which SYBR® Green master mix is right for you?
SuperScript® reverse transcriptase selection guide
The SuperScript® family of RT enzymes provides higher yields of full-length cDNA for more complete representation of input RNA compared to standard reverse transcriptases. Choose the right enzyme for your application.