Agarose gel electrophoresis is an important technique when examining PCR products, genotyping or preparing probes, and is commonly used to identify, quantify, and purify PCR fragments.
Life Technologies offers convenient reagents for agarose gel electrophoresis including hassle-free precast E-Gel® agarose gels, safe and sensitive DNA stains, DNA ladders, and UltraPure™ reagents to cast agarose gels yourself.
Below are the basic steps of this technique and some tips for success.
Agarose gel electrophoresis: basic steps
|Step 1: Prepare UltraPure™ reagents
UltraPure™ agarose powder and UltraPure™ electrophoresis buffer (TBE or TAE) give you consistent quality at a great price.
|Step 2: Load DNA samples
Load your samples along with a ready to load TrackIt™ DNA Ladder for maximum resolution and ease of tracking.
|Step 3: Run gel with PowerEase® power supply
Run gel with easy-to-use, programmable PowerEase® Power Supply.
|Step 4: Stain gel in tray
Ethidium bromide (EtBr) stain offers sensitive nucleic acid detection, but is both a mutagen and carcinogen. SYBR® Safe DNA stain offers similar detection sensitivity and is designed to be a safer alternative to EtBr.
|Step 5: Visualize with E-Gel® Imager
Visualize your DNA bands with the high performance E-Gel® imaging system. Analyze and quantify your DNA sample with easy-to-use GelQuant™ Express analysis software and GelCapture™ Acquisition software, included with imager.
What products are used during the visualize step?
E-Gel® agarose gel electrophoresis and E-Gel® Imager systems
Tired of pouring hot agarose and want convenience and time-savings without sacrificing performance? Try the hassle-free, precast E-Gel® agarose gel electrophoresis and E-Gel® Imager systems.
E-Gel® agarose gel electrophoresis system
E-Gel® agarose gel electrophoresis system is a complete bufferless system for analyzing DNA and RNA samples.
E-Gel® CloneWell™ gels allow you to extract DNA bands of interest from your gel without any purification steps, helping save time and effort and improving your cloning efficiency. Attend the webinar on E-Gel® CloneWell™ gels and learn how this innovative method can maximize the amount of DNA you recover.
E-Gel® Imager is an economical, space-saving gel documentation system with the performance of a complex imaging system. Watch the video on how E-Gel® Imager has revolutionized gel documentation.