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Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments. With PCR enzymes you know and trust such as Applied Biosystems AmpliTaq and AmpliTaq Gold, and Invitrogen Platinum Taq and Platinum SuperFi DNA polymerases, we have what it takes for successful PCR.

PCR types

To increase specificity and yield

  Platinum II Taq
Hot-Start DNA Polymerase

AmpliTaq Gold DNA Polymerase
AmpliTaq Gold 360 DNA Polymerase
Hot-start technology Antibody Chemical Chemical
Universal annealing protocol Yes No No
Speed 15 sec/kb 60 sec/kb 60 sec/kb
Flexible extension step* Yes No No
Inhibitor resistance Yes No No
Enhanced specificity Yes Yes Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 5 kb
GC-rich format Yes No Yes
Activation time 30 sec to 2 min 10 min 5–10 min
Master mix/Super mix format Colorless
Green**
  Colorless
Stand-alone enzyme Colorless
Green***
Colorless Colorless
* The extension step can be extended up to 60 sec/kb without the effect of specificity.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
*** Green buffer available as separate item for use with stand-alone enzyme.

For when sequence accuracy is critical

  Platinum SuperFi DNA Polymerase
AccuPrime Taq DNA Polymerase High Fidelity
Platinum Taq DNA Polymerase High Fidelity
Fidelity (vs. Taq) >100x 9x 6x
Hot-start for enhanced specificity Yes Yes Yes
Amplicon size up to 20 kb* up to 20 kb up to 20 kb
Overhang Blunt 3′ A/Blunt 3′ A/Blunt
GC-rich format Yes    
Master mix/super mix format Colorless
Green**
  Colorless
Stand-alone enzyme Colorless
Green**
Colorless Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For robust yields of templates longer than 10 kb

  Platinum SuperFi DNA Polymerase
Amplicon size Up to 20 kb*
Fidelity (vs. Taq) >100x
Hot-start for enhanced specificity Yes
Master mix/SuperMix Colorless
Green**
Stand-alone enzyme Colorless
Green**
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify multiple targets in one reaction

  Platinum SuperFi
DNA Polymerase

Platinum Multiplex
PCR Master Mix

No. of amplicons in single reaction Up to 15-plex Up to 20-plex
Amplicon size Up to 2.5 kb Up to 2.5 kb
Hot-start for enhanced specificity Yes Yes
Fidelity (vs. Taq) >100x 1x
Master mix/super mix format Colorless
Green*
Colorless
Stand-alone enzyme Colorless
Green*
 
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Application note

Other reagents for multiplex PCR

For accurate detection and identification of microbiome composition by 16S rRNA genes

  Platinum II Taq
DNA Polymerase

Platinum Taq
DNA Polymerase,
DNA-free

Platinum SuperFi
DNA Polymerase

Bacterial gDNA copy per enzyme unit ≤1 copy ≤0.01 copy ≤1 copy
Human gDNA copy per enzyme unit ≤0.2 copy ≤0.001 copy ≤0.2 copy
Speed 15 sec/kb 60 sec/kb 15–30 sec/kb
Inhibitor resistance Yes No Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 20 kb
Fidelity (vs. Taq) 1x 1x >100x
GC-rich format Yes No Yes
Hot-start for enhanced specificity Yes Yes Yes
Universal primer annealing Yes No No
Master mix format Colorless
Green*
  Colorless
Green*
Stand-alone enzyme Colorless
Green*
Colorless Colorless
Green*
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Flyer

Application note

Poster

For everyday PCR amplification

  Taq DNA Polymerase
AmpliTaq DNA Polymerase
Amplicon size Up to 5 kb Up to 5 kb
Activation time Immediate Immediate
Product overhang 3′ A 3′ A
Exonuclease activity 5′→ 3′ 5′→ 3′
Master mix/SuperMix format Colorless  
Stand-alone enzyme Colorless (recombinant)
Colorless (native)*
Colorless
* Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified.

Other PCR enzymes

You can also use these enzymes for everyday PCR amplification:

To increase specificity and yield

  Platinum II Taq
Hot-Start DNA Polymerase

AmpliTaq Gold DNA Polymerase
AmpliTaq Gold 360 DNA Polymerase
Hot-start technology Antibody Chemical Chemical
Universal annealing protocol Yes No No
Speed 15 sec/kb 60 sec/kb 60 sec/kb
Flexible extension step* Yes No No
Inhibitor resistance Yes No No
Enhanced specificity Yes Yes Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 5 kb
GC-rich format Yes No Yes
Activation time 30 sec to 2 min 10 min 5–10 min
Master mix/Super mix format Colorless
Green**
  Colorless
Stand-alone enzyme Colorless
Green***
Colorless Colorless
* The extension step can be extended up to 60 sec/kb without the effect of specificity.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
*** Green buffer available as separate item for use with stand-alone enzyme.

For when sequence accuracy is critical

  Platinum SuperFi DNA Polymerase
AccuPrime Taq DNA Polymerase High Fidelity
Platinum Taq DNA Polymerase High Fidelity
Fidelity (vs. Taq) >100x 9x 6x
Hot-start for enhanced specificity Yes Yes Yes
Amplicon size up to 20 kb* up to 20 kb up to 20 kb
Overhang Blunt 3′ A/Blunt 3′ A/Blunt
GC-rich format Yes    
Master mix/super mix format Colorless
Green**
  Colorless
Stand-alone enzyme Colorless
Green**
Colorless Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For robust yields of templates longer than 10 kb

  Platinum SuperFi DNA Polymerase
Amplicon size Up to 20 kb*
Fidelity (vs. Taq) >100x
Hot-start for enhanced specificity Yes
Master mix/SuperMix Colorless
Green**
Stand-alone enzyme Colorless
Green**
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify multiple targets in one reaction

  Platinum SuperFi
DNA Polymerase

Platinum Multiplex
PCR Master Mix

No. of amplicons in single reaction Up to 15-plex Up to 20-plex
Amplicon size Up to 2.5 kb Up to 2.5 kb
Hot-start for enhanced specificity Yes Yes
Fidelity (vs. Taq) >100x 1x
Master mix/super mix format Colorless
Green*
Colorless
Stand-alone enzyme Colorless
Green*
 
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Application note

Other reagents for multiplex PCR

For accurate detection and identification of microbiome composition by 16S rRNA genes

  Platinum II Taq
DNA Polymerase

Platinum Taq
DNA Polymerase,
DNA-free

Platinum SuperFi
DNA Polymerase

Bacterial gDNA copy per enzyme unit ≤1 copy ≤0.01 copy ≤1 copy
Human gDNA copy per enzyme unit ≤0.2 copy ≤0.001 copy ≤0.2 copy
Speed 15 sec/kb 60 sec/kb 15–30 sec/kb
Inhibitor resistance Yes No Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 20 kb
Fidelity (vs. Taq) 1x 1x >100x
GC-rich format Yes No Yes
Hot-start for enhanced specificity Yes Yes Yes
Universal primer annealing Yes No No
Master mix format Colorless
Green*
  Colorless
Green*
Stand-alone enzyme Colorless
Green*
Colorless Colorless
Green*
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Flyer

Application note

Poster

For everyday PCR amplification

  Taq DNA Polymerase
AmpliTaq DNA Polymerase
Amplicon size Up to 5 kb Up to 5 kb
Activation time Immediate Immediate
Product overhang 3′ A 3′ A
Exonuclease activity 5′→ 3′ 5′→ 3′
Master mix/SuperMix format Colorless  
Stand-alone enzyme Colorless (recombinant)
Colorless (native)*
Colorless
* Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified.

Other PCR enzymes

You can also use these enzymes for everyday PCR amplification:

  • Extra PCR buffers, when available, can be ordered from their respective enzyme pages provided above.
  • dNTPs are also available in a variety of concentrations and formats.

Need DNA-free DNA polymerases for microbial research or sensitive PCR-based assays? Find out more

Videos

Resources

  • PCR education
    Learn about the history of PCR, setup considerations, importance of DNA polymerase choice, common methods and applications, and troubleshooting.
  • Tm calculator
    Use our application to calculate the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases.
  • Molecular biology webinars
    View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR and cloning.
  • OEM & customized PCR solutions
    Discover customizable manufacturing solutions, product labeling, and packaging capabilities for your specific requirements.
Support
  • PCR and cDNA synthesis support center
    Find tips, troubleshooting help, and resources for your end-point PCR and cDNA applications. 
  • Product support documentation
    Search for manuals, protocols, Material Safety Data Sheets, product literature and certificates by catalog number or product name.
  • Contact us
    Email or call our Technical Application Scientists for additional questions regarding PCR enzymes and master mixes.