Hot-Start PCR Enzymes
During PCR reaction setup, prior to the start of thermal cycling, DNA polymerases commonly exhibit some synthesis activity. This activity can lead to mispriming and the formation of primer dimers, which can compromise the specificity and yield of the reaction. We've developed a range of hot-start enzymes—including Platinum™ Taq and AmpliTaq Gold™ enzymes—designed to minimize pre-cycling polymerase activity for more efficient, specific PCR.
Which hot-start PCR enzyme is right for you?
|Platinum™ Taq DNA polymerase
|AmpliTaq Gold™ DNA polymerase
|AmpliTaq Gold™ 360 DNA polymerase
|Amplicon size||Up to 5 kb||Up to 5 kb||Up to 5 kb|
|Activation time||30 seconds to 2 minutes||10 minutes||5-10 minutes|
|Master mix available||Yes||Yes|
|Direct gel loading||Yes|