During PCR reaction setup, prior to the start of thermal cycling, DNA polymerases commonly exhibit some synthesis activity. This activity can lead to mispriming and the formation of primer dimers, which can compromise the specificity and yield of the reaction. We've developed a range of hot-start enzymes—including AmpliTaq Gold® and Platinum® Taq enzymes—designed to minimize pre-cycling polymerase activity for more efficient, specific PCR.

Which hot-start PCR enzyme is right for you?

Chemically modified hot-start DNA polymerase Chemically modified hot-start master mix Antibody-mediated hot-start DNA polymerase Antibody-mediated hot-start master mix
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  AmpliTaq Gold®
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AmpliTaq Gold® 360 Master Mix Platinum® Taq
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Platinum® PCR SuperMix
Enhanced specificity
Amplicon size Up to 5 kb Up to 5 kb Up to 10 kb Up to 5 kb
GC-rich format
Activation time 10 minutes 5-10 minutes 30 seconds 30 seconds
Master Mix/Super Mix format Yes Yes