During PCR reaction setup, prior to the start of thermal cycling, DNA polymerases commonly exhibit some synthesis activity. This activity can lead to mispriming and the formation of primer dimers, which can compromise the specificity and yield of the reaction. We've developed a range of hot-start enzymes—including Platinum™ Taq and AmpliTaq Gold™ enzymes—designed to minimize pre-cycling polymerase activity for more efficient, specific PCR.

Which hot-start PCR enzyme is right for you?

  Platinum™ Taq DNA polymerase
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AmpliTaq Gold™ DNA polymerase
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AmpliTaq Gold™ 360 DNA polymerase
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Hot-start technology Antibody Chemical Chemical
Enhanced specificity
Amplicon size Up to 5 kb Up to 5 kb Up to 5 kb
GC-rich format
Activation time 30 seconds to 2 minutes 10 minutes 5-10 minutes
Master mix available Yes Yes
Direct gel loading Yes