Invitrogen™ Platinum™ Taq DNA Polymerase is a hot-start enzyme trusted by researchers for robust, reliable amplification in PCR applications such as genotyping, gene expression profiling and next-generation sequencing (NGS).

  • High specificity and increased yields with antibody-mediated hot-start PCR
  • Versatile formulation for broad range of amplicons
  • Convenient room-temperature reaction set-up
  • Versions with green buffer enable direct gel loading

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Platinum Taq DNA Polymerase products

  Stand-alone enzyme 2x master mix
Without direct loading dye Colorless Colorless*
With direct loading dye Green Green*

* 2x master mixes are the recommended product formats to upgrade from PlatinumTM PCR SuperMix and PlatinumTM Blue PCR SuperMix.


Features of Platinum Taq DNA Polymerases

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. Platinum Taq DNA Polymerase is provided in an inactive form, due to specific binding of the inhibitor. The polymerase is activated in a temperature-dependent manner (at 94ºC) during the start of PCR. Once dissociated from the inhibitor, the Taq DNA polymerase regains its full activity.

Video: A true hot-start Taq polymerase



Invitrogen™ Platinum™ Taq Hot Start products offer the convenience of a green buffer with a density reagent and two tracking dyes for direct gel loading of PCR products. The green buffer eliminates tedious dye addition steps prior to gel loading and is compatible with downstream applications including DNA sequencing, ligation and restriction digestion.

Simplified PCR workflow. Convenient Platinum Hot Start master mixes helps reduce the number of steps during PCR reaction setup. Green buffer enables direct gel loading of PCR products, eliminating tedious steps of dye addition. Left lane of gel image shows reaction mixture with green buffer prior to electrophoresis. Right lanes of gel show yellow and blue dye migration following 5 and 15 minutes of electrophoresis.

Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. Hot-start PCR enables a greater yield of the target amplicon with the added convenience of room-temperature PCR setup.

Platinum Taq Hot Start DNA Polymerase enables high specificity and robust yields. Seven fragments, 500 to 800 bp in length and of varying GC content, were amplified from 30 ng of human gDNA using both colorless (–) and green (+) PCR buffers.

Even low-abundance DNA templates can be detected for accurate results. The high sensitivity of Platinum Taq Hot Start PCR reagents is a great advantage in experiments where there is a limited amount of starting material or the target DNA is in low concentration in the sample.

High sensitivity and reliable amplification from low amounts of input DNA. Amplification of a 222 bp human gDNA fragment from DNA input ranging from 0.5 ng (~150 copies) to 1 μg using Invitrogen™ Platinum™ Green Hot Start PCR 2X Master Mix. Each reaction was performed in duplicate. NTC = no-template control.

 

Formulation of Invitrogen™ Platinum™ Taq Hot Start 2x Master Mixes allow for amplification of versatile range of targets.

Platinum Hot Start PCR 2X Master Mixes are supplied with a separate vial of a special GC enhancer formulated for specific amplification and improved yields of targets with difficult-to-amplify, high-GC content.

Platinum Hot Start PCR Master Mix enables robust amplification of GC-rich DNA. 700–750 bp GC-rich fragments were amplified from 30 ng of human gDNA using Platinum Hot Start PCR 2X Master Mix supplemented with GC Enhancer (P) or hot-start Taq PCR master mixes from other vendors (G, O).

Platinum Taq Hot Start 2X Master Mixes are also formulated to amplify a broad range of target lengths.

Amplification across versatile range of target lengths. 1.2 kb, 1.8 kb, 2.0 kb, 3.9 kb, 4.5 kb and 5 kb long fragments (lanes 1-6) were amplified from 100 ng of human gDNA using Platinum Green Hot Start PCR 2X Master Mix.

 

 

Extended stability of the Platinum Taq DNA Polymerase hot-start enzyme at room temperature enables high-throughput applications.

Platinum Taq DNA Polymerase stability at room temperature. Amplification of 2 kb beta-globin gene fragment from human gDNA  (0.2, 2 and 20 ng) was  performed using Platinum Taq DNA Polymerase with colorless (left panel) or green (right panel) buffer. PCR reactions were set up and incubated at room temperature 0, 24, and 72 h before loading in the thermal cycler. Even after 72 h of room temperature incubation, highly specific amplification is enabled with stable antibody-mediated hot-start technology. Each reaction was performed in duplicate. NTC = no template control.

Are you using a “warm-start” Taq vs. a true “hot-start” Taq polymerase?

In heat-activation tests, polymerases were heat-treated at 94°C for 2 minutes to dissociate the antibodies from the polymerases. Polymerase activity was measured at 60°C (constant) for 60 minutes. The side-by-side comparison demonstrates that the activity of Platinum Taq DNA Polymerase is almost completely blocked in the non–heat-activated condition, whereas competitor T’s hot-start enzyme has a high level of residual polymerase activity.

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