Isolated from the thermophilic bacterium Thermus aquaticus, Taq DNA polymerase is one of the best-known thermostable DNA polymerases used in PCR amplification of DNA targets. The native enzyme is purified from Thermus aquaticus YT1. The recombinant enzyme is a cloned version that is purified from E. coli. Therefore, the native Taq DNA polymerase is often preferred for amplification of bacterial DNA sequences homologous to E. coli sequences.
Since the first report on PCR using Taq DNA polymerase, better-performing DNA polymerases are continually being developed to meet the needs of increasingly challenging research applications. The enzymes listed below help improve PCR performance in a variety of aspects such as sensitivity, specificity, accuracy, and inhibitor tolerance.
|Sensitivity (yield)||Specificity (hot start)||Accuracy (vs. Taq fidelity)||Inhibitor tolerance||GC-rich template||Amplification range||Low residual bacterial gDNA|
|(e.g., for low-abundance targets)||(e.g., for genotyping)||(e.g., for cloning, mutagenesis)||(e.g., for clinical research samples)||(e.g., for promoter sequences)||(e.g., for long DNA targets)||(e.g., for microbiome studies)|
|Platinum II Taq DNA Polymerase||+++||+++||1x||+++||+++||5 kb||≤1 copy/50 μL rxn|
|Platinum SuperFi II DNA Polymerase||+++||+++||>300x||+++||+++||20 kb||≤1 copy/50 μL rxn|
|Platinum Taq DNA Polymerase, DNA-free||++||+++||1x||+||+||5 kb||≤0.01 copy/50 μL rxn|
|Standard Taq DNA polymerase||+||+||1x||+||+||5 kb||Not tested|
For Research Use Only. Not for use in diagnostic procedures.