Invitrogen™ Elongase™ is a mixture of Taq DNA Polymerase (Recombinant) and Pyrococcus sp. GB-D thermostable DNA polymerase. The ratio of the two thermostable polymerases is such that the processivity and activity of Taq and the 3′-5′ exonuclease of the Pyrococcus sp. GB-D polymerase allow for the amplification of fragments up to 30 kb (up to 30 kb with lambda sequence and up to 20 kb for a single copy sequence in genomic DNA) and the greater amplification of smaller fragments. Amplification using Taq DNA polymerase is generally limited to DNA template less than 5 kb. This limitation is believed to be largely due to the inability of Taq DNA Polymerase, which lacks proofreading 3′-5′ exonuclease activity to correct nucleotide misincorporations and continue primer elongation. Including a small amount of proofreading enzyme edits the nascent strand to allow subsequent polymerization by Taq.

Components Elongase Enzyme Mix Elongase Enzyme Mix
Cat. No. 10480010 10480028
Enzyme Mix 100 500
5X Buffer A 1.0 mL 5 x 1.0 mL
5X Buffer B 1.0 mL 5 x 1.0 mL