AccuPrime PCR Systems

Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime technology is available in Taq, Pfx, and GC-Rich formulations.

Overview of AccuPrime technology

The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with antibodies that are specific to the DNA polymerase, inhibiting its activity at room temperature and providing an automatic hot start.

How AccuPrime technology works
How AccuPrime technology works.

Comparison of Platinum and AccuPrime DNA polymerases

Properties of Platinum DNA polymerases and AccuPrime DNA polymerases are compared for their use in high-fidelity PCR, hot-start PCR, and GC-rich PCR.

  Platinum SuperFi DNA Polymerase AccuPrime Taq DNA Polymerase, High Fidelity AccuPrime Pfx DNA Polymerase
Fidelity (vs. Taq) >100x 9x 26x
Hot-start modification      
Amplification range 20 kb* ≤20 kb (with Buffer II) ≤12 kb
Amplicon overhang Blunt 3′A and blunt Blunt
GC-rich format   No No
Inhibitor tolerance   No No
DNA synthesis rate 15–30 sec/kb 60 sec/kb 60 sec/kb
Residual bacterial gDNA ≤1 copy/enzyme unit Not tested Not tested
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
One buffer
Product formats Stand alone:
Colorless
Green
Master mix:
Colorless
Green
Stand alone:
Colorless
Stand alone:
Colorless

* Amplification up to 40 kb is possible but may require additional optimization of reaction conditions and primer design
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

  Platinum II Taq DNA Polymerase AccuPrime Taq DNA Polymerase System
Hot-start modification    
Fidelity (vs. Taq) 1x 2x
Amplification range ≤5 kb ≤5 kb
Inhibitor tolerance   No
DNA synthesis rate 15 sec/kb 60 sec/kb
Amplicon overhang 3′A 3′A
Universal primer annealing   No
Residual bacterial gDNA ≤1 copy/enzyme unit Not tested
GC-rich format   No
Multiplexing Up to 15 Up to 20
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
Product formats Stand alone:
 Colorless
Master mix:
 Colorless
 Green
Stand alone:
 Colorless

† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

  Platinum SuperFi DNA Polymerase Platinum II Taq DNA Polymerase AccuPrime GC-Rich DNA Polymerase
GC-rich amplification >65% GC >65% GC >65% GC
Hot-start modification     No
Fidelity (vs. Taq) >100x 1x 2x
Amplification range 20 kb* ≤5 kb ≤5 kb
Amplicon overhang Blunt 3′A Blunt
Inhibitor tolerance     No
DNA synthesis rate 15–30 sec/kb 15 sec/kb 60 sec/kb
Universal primer annealing No   No
Residual bacterial gDNA ≤1 copy/enzyme unit ≤1 copy/enzyme unit Not tested
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
One optimized buffer
(GC enhancer supplied in a separate vial)
Two-buffer system
(based on GC%)
Product formats Stand alone:
 Colorless
 Green
Master mix:
 Colorless
 Green
Stand alone:
Colorless

Master mix:
 Colorless
 Green
Stand alone:
 Colorless

* Amplification up to 40 kb is possible but may require additional optimization of reaction conditions and primer design
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

  Platinum SuperFi DNA Polymerase AccuPrime Taq DNA Polymerase, High Fidelity AccuPrime Pfx DNA Polymerase
Fidelity (vs. Taq) >100x 9x 26x
Hot-start modification      
Amplification range 20 kb* ≤20 kb (with Buffer II) ≤12 kb
Amplicon overhang Blunt 3′A and blunt Blunt
GC-rich format   No No
Inhibitor tolerance   No No
DNA synthesis rate 15–30 sec/kb 60 sec/kb 60 sec/kb
Residual bacterial gDNA ≤1 copy/enzyme unit Not tested Not tested
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
One buffer
Product formats Stand alone:
Colorless
Green
Master mix:
Colorless
Green
Stand alone:
Colorless
Stand alone:
Colorless

* Amplification up to 40 kb is possible but may require additional optimization of reaction conditions and primer design
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

  Platinum II Taq DNA Polymerase AccuPrime Taq DNA Polymerase System
Hot-start modification    
Fidelity (vs. Taq) 1x 2x
Amplification range ≤5 kb ≤5 kb
Inhibitor tolerance   No
DNA synthesis rate 15 sec/kb 60 sec/kb
Amplicon overhang 3′A 3′A
Universal primer annealing   No
Residual bacterial gDNA ≤1 copy/enzyme unit Not tested
GC-rich format   No
Multiplexing Up to 15 Up to 20
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
Product formats Stand alone:
 Colorless
Master mix:
 Colorless
 Green
Stand alone:
 Colorless

† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

  Platinum SuperFi DNA Polymerase Platinum II Taq DNA Polymerase AccuPrime GC-Rich DNA Polymerase
GC-rich amplification >65% GC >65% GC >65% GC
Hot-start modification     No
Fidelity (vs. Taq) >100x 1x 2x
Amplification range 20 kb* ≤5 kb ≤5 kb
Amplicon overhang Blunt 3′A Blunt
Inhibitor tolerance     No
DNA synthesis rate 15–30 sec/kb 15 sec/kb 60 sec/kb
Universal primer annealing No   No
Residual bacterial gDNA ≤1 copy/enzyme unit ≤1 copy/enzyme unit Not tested
Buffer system One optimized buffer
(GC enhancer supplied in a separate vial)
One optimized buffer
(GC enhancer supplied in a separate vial)
Two-buffer system
(based on GC%)
Product formats Stand alone:
 Colorless
 Green
Master mix:
 Colorless
 Green
Stand alone:
Colorless

Master mix:
 Colorless
 Green
Stand alone:
 Colorless

* Amplification up to 40 kb is possible but may require additional optimization of reaction conditions and primer design
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

AccuPrime DNA polymerase products

The following table summarizes AccuPrime DNA polymerases and formats available for PCR.

  AccuPrime Taq DNA Polymerase, High Fidelity AccuPrime Pfx DNA Polymerase AccuPrime GC-Rich DNA Polymerase AccuPrime Taq DNA Polymerase System
DNA polymerase(s) Blend of Taq DNA polymerase and proofreading GB-D enzyme from Pyrococcus species Recombinant KOD enzyme from Thermococcus species DNA polymerase cloned from Pyrolobus fumarius AccuPrime Taq DNA polymerase
Hot-start modification     No  
Enhanced specificity with AccuPrime accessory proteins        
Fidelity
(vs. Taq)
9x 26x 2x 2x
Amplicon overhang 3′A and blunt Blunt Blunt 3′A
Product formats Stand-alone enzyme with the two-buffer system:
Buffer I for plasmids, cDNA, and λ DNA;
Buffer II for gDNA.
Stand-alone enzyme Stand-alone enzyme with the two-buffer system:
Buffer A for GC-rich gDNA targets;
Buffer B for non–GC-rich gDNA, cDNA, plasmids, and λ DNA.
Stand-alone enzyme with the two-buffer system:
Buffer I for small gDNA amplicons (≤200 bp), cDNA, and plasmids;
Buffer II for gDNA (200 bp–4 kb).

Ordering information

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