qPCR reactions use the same components as standard end-point PCR reactions, with the addition of a fluorescent label that creates a signal during the PCR reaction. The products for qPCR are fluorescently labeled using one of two main strategies:
- Applied Biosystems™ TaqMan® fluorogenic probes—target-specific oligonucleotides that produce a fluorescent signal only when the target DNA is amplified during qPCR.
- Applied Biosystems™ SYBR™ Green I dye—binds to double-stranded DNA and emits fluorescence only when bound.
There are many factors that help determine which labeling chemistry is right for your applications. Our TaqMan Chemistry vs. SYBR Chemistry for Real-Time PCR selection guide can help you determine which chemistry to use.