I am having problems with...


Abnormal Amplification

Your curves are sigmoidal or amplification occurs later than you expected.

NTC Positive Amplification

You have an amplification product in your no template control (NTC).

No Amplification

You have no detectable PCR product.

Poor PCR Efficiency

If the slope of your standard curve is below -3.6, you have poor PCR efficiency.

Normal Amplification Curve Example

Your amplification curve should look something like this