Instrument calibration

To ensure optimal performance of your thermal cycler or sequence detection system, Applied Biosystems strongly recommends that you regularly calibrate the system. Calibration schedules vary by instrument; refer to the table below for the types of calibration and appropriate intervals.

Instrument calibration intervals

Calibration Type Instrument Interval
ROI (Spatial) 7300/7500/7500 Fast Real-time PCR Systems StepOne™ / StepOnePlus™ Systems Every 6 months or As needed to verify the instrument’s performance
Background 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems Every month or Before performing a pure dye calibration or When installing an uncalibrated block
Pure dye spectra 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems Every 6 months or When installing an uncalibrated block
Instrument verification 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems Every 6 months or As needed to verify the instrument’s performance