Get a master mix you can trust from a leader in qPCR

Optimized real-time PCR (qPCR) analysis enables sensitive and specific quantification of nucleic acids. Reagent selection plays a critical role in any qPCR or RT-qPCR protocol to help ensure optimal performance and reliable results. Set your analysis up for success by choosing an Applied Biosystems real-time PCR master mix or reagent.


Real-time PCR master mixes, sometimes referred to as "mastermixes", "super mixes", or "ready mixes", are concentrated, pre-mixed solutions that contain many components required for a qPCR reaction, including buffer, enzymes, and dNTPs. With over 25 years of experience innovating real-time PCR reagents, Thermo Fisher Scientific has developed optimized master mixes for almost any qPCR workflow. Our reagent product families (TaqMan, TaqPath, and SYBR) offer master mix formulations tailored to meet the unique requirements of different applications, such as gene expression,  genotyping, and pathogen detection.

Real-Time PCR reagent & master mix options

Gene expression analysis

For accurate detection and quantification of gene expression targets, view our recommended TaqMan and SYBR Green master mixes for gene expression analysis.

Genotyping and genetic variation analysis

Analyze single nucleotide polymorphisms (SNPs), copy number variation (CNV), and other genetic variations accurately and efficiently.

Pathogen and microbial detection

Detect bacterial and viral targets with high sensitivity and specificity.

Master mix resources

Find product literature, FAQs, and educational materials related to qPCR master mixes.

Other reagents & kits

Explore more products including HRM master mixes, exogenous controls, sample-to-results kits, instrument calibration and verification kits, and Lyo-Ready master mixes that support lyophilized assay developers.

TaqPath master mixes

Explore master mix products for mid- to late-stage biopharmaceutical development, clinical applications, and molecular diagnostic test development.

TaqMan and SYBR Green master mixes are For Research Use Only. Not for use in diagnostic procedures. TaqPath master mixes are general purpose reagents labeled For Laboratory Use.