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Supporting confident qPCR and RT-qPCR results across research workflows
Applied Biosystems probe-based qPCR and RT-qPCR master mixes support a broad range of research applications across academic, government, and biopharma laboratories. Our portfolio includes TaqMan master mixes designed to help researchers achieve reliable results across diverse workflows, targets, and experimental requirements, as well as TaqPath master mixes for advanced applications such as higher-order multiplexing, SNP genotyping, and automated workflows
Reliable results across qPCR and RT-qPCR applications
From gene expression studies and biomarker verification to microbial detection and pathway analysis, researchers rely on qPCR and RT-qPCR to generate relevant genetic insights. Applied Biosystems probe-based master mixes are designed to support reliable performance across diverse research applications and workflow requirements.
* Data shown are illustrative of product performance. Results may vary depending on assay design, target, sample type, instrument, and workflow.
Probe qPCR and RT-qPCR master mix selection guide
Applied Biosystems probe-based qPCR and RT-qPCR master mixes are designed to support different research applications and workflow requirements. Use the guides below to identify the TaqMan or TaqPath master mix best suited to your research application, sample type, and experimental needs.
Which TaqMan master mix should I use?
TaqMan master mixes are designed to support common qPCR and RT-qPCR research applications, including gene expression analysis and microbial detection. Use the guide below to identify the TaqMan master mix best suited to your application and workflow requirements.
Application |
Recommended product |
Key features |
|---|---|---|
2-Step Gene Expression |
TaqMan Fast Advanced Master Mix |
cDNA input, multiplex gene expression, low-expression quantification, benchtop stability |
1-Step Gene Expression |
TaqMan Fast Virus 1-Step Master Mix |
RNA input, one-step RT-qPCR, multiplex assays, inhibitor tolerance |
Microbial Detection (RNA Targets) |
TaqMan Fast Virus 1-Step Master Mix |
RNA targets, one-step RT-qPCR, inhibitor tolerance, complex sample types |
miRNA Analysis |
TaqMan Fast Advanced Master Mix |
For standard and advanced TaqMan miRNA chemistry |
When should I choose a TaqPath Master Mix for my research workflow?
TaqPath master mixes can help support research workflows with specialized requirements, including higher-order multiplexing, extended benchtop stability, automation compatibility, and complex sample types. Use the guide below to identify if a TaqPath master mix is suited to your research application and workflow requirements.
Application or workflow requirement |
Recommended product |
Key features |
|---|---|---|
1-Step RT-qPCR requiring high-order multiplex |
TaqPath DuraPlex 1-Step RT-qPCR Master Mix |
Up to 6-plex assays, benchtop stability, automated workflows |
1-Step RT-qPCR requiring extended benchtop stability |
TaqPath DuraPlex 1-Step RT-qPCR Master Mix |
Extended benchtop stability, automated workflows, multiplex assays |
Microbial detection (DNA targets) |
TaqPath BactoPure Microbial Detection Master Mix |
DNA targets, inhibitor-rich samples, low-level microbial detection |
SNP genotyping |
TaqPath ProAmp Master Mix |
Genomic DNA or crude cell lysate input, reliable calls, multiplex genotyping |
Copy number variation analysis |
TaqPath ProAmp Master Mix |
Genomic DNA input, copy number determination, high-throughput analysis |
qPCR and RT-qPCR master mixes
Need OEM or custom solutions?
Need support beyond off-the-shelf reagents? Thermo Fisher Scientific offers OEM support, custom manufacturing, and commercialization services.
Frequently asked questions about TaqMan Master Mixes
Choose TaqMan Fast Advanced Master Mix for 2-step gene expression workflows using cDNA generated from a separate reverse transcription reaction. Choose TaqMan Fast Virus 1-Step Master Mix for 1-step RT-qPCR workflows that use RNA as the input. The appropriate choice depends on your sample type, workflow design, and experimental requirements.
TaqMan Fast Advanced Master Mix is designed for 2-step qPCR workflows using cDNA generated from a separate reverse transcription reaction. TaqMan Fast Virus 1-Step Master Mix combines reverse transcription and qPCR in a single reaction, enabling 1-step RT-qPCR directly from RNA. Choose the master mix based on whether your workflow starts with cDNA or RNA.
For low-abundance targets, choose TaqMan Fast Advanced Master Mix for 2-step gene expression workflows using cDNA. Choose TaqMan Fast Virus 1-Step Master Mix for 1-step RT-qPCR workflows using RNA. Both master mixes are designed to support sensitive detection and quantification of low-level targets, with selection based primarily on workflow design and sample input.
Yes. TaqMan Fast Virus 1-Step Master Mix is designed for 1-step RT-qPCR workflows and may be used for microbial detection research requiring RNA target detection. Researchers can evaluate performance using their own assays, samples, and instruments.
Yes. TaqMan Fast Advanced Master Mix and TaqMan Fast Virus 1-Step Master Mix support multiplex qPCR and RT-qPCR workflows, enabling detection of multiple targets within a single reaction when used with appropriately designed assays.
TaqMan master mixes are compatible with a broad range of real-time PCR instruments. Refer to individual product pages for product-specific instrument compatibility information and workflow recommendations.
TaqMan master mixes can be evaluated using your own assays, samples, and qPCR instruments to assess performance under your experimental conditions. Depending on your region and product availability, evaluation options or promotional programs may be available.
Order TaqMan Master Mixes
TaqMan Fast Advanced Master Mix
TaqMan Fast Virus 1-Step Master Mix
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX)
For Research Use Only. Not for use in diagnostic procedures.