TaqMan Fast Advanced Master Mix

High sensitivity, accuracy, and dynamic range, even with challenging targets

Applied Biosystems TaqMan Fast Advanced Master Mix provides excellent performance with superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode. This next-generation master mix employs our novel AmpliTaq Fast DNA Polymerase, giving you stability at room temperature for up to 72 hours. Additionally, this master mix offers reduced run times, is optimized for multiplexing, and is ideal for any gene expression experiment or quantitative analysis, including pathogen detection, viral load quantitation, microRNA quantification, and microarray verification.

 

TaqMan Fast Advanced Master Mix can be used with any DNA target, including complementary DNA (cDNA) or genomic DNA (gDNA). It can also be used in the second step of a two-step real-time RT-PCR protocol for RNA quantitation experiments. A cDNA template can be generated from RNA using one of our reverse transcription kits prior to real-time PCR.

Features

Key features of the TaqMan Fast Advanced Master Mix include:

Excellent performance

Benchmarked against other standard and fast master mixes to consistently deliver best-in-class sensitivity, accuracy, dynamic range, and specificity.

Optimized for multiplexing

Validated for duplexing with exogenous and endogenous internal positive control assays.

Reduced run times on fast and standard instrumentation

Optimized on fast instruments and fast cycling conditions on standard instruments.

Engineered for enhanced benchtop stability

Stable at room temperature for up to 72 hours in preassembled reactions.

Seamlessly transitions into your experiments

Verified with TaqMan Assays for gene expression and miRNAs, and with TaqMan array microfluidic cards.

Sample data

72 hour benchtop stability

The enhanced stability of TaqMan Fast Advanced Master Mix assures users of high-throughput liquid handling systems that the results on the first plate will mimic those of the last plate. It also adds overall convenience to your workflow, as you no longer need to run your plates immediately upon assembly.

Benchtop stability of TaqMan Fast Advanced Master Mix

This B2M TaqMan gene expression assay was run upon assembly (time 0) (A) and after 72 hr of incubation at 30°C (B). The results after 72 hr show excellent PCR efficiency, R2 values, and Ct when compared to time 0.

Data figures showing benchtop stability of TaqMan Fast Advanced Master Mix

High PCR efficiency in both simplex and duplex

For added confidence in the results in every well, TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC).

TaqMan Fast Advanced Master Mix is optimized for multiplexing with exogenous or endogenous controls

Results are shown for ß-actin (gene name ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions. The duplex reactions include the single-target ACTB and either a constant quantity of exogenous target (Figure A) or a relative quantity of endogenous target (Figure B). Orange simplex data points are almost hidden behind the green duplex data points, confirming nearly identical results in both modes.

TaqMan Fast Advanced Master Mix is optimized for multiplexing with exogenous or endogenous controls

Excellent PCR linearity over a 6-log range of input template

The formulation of TaqMan Fast Advanced Master Mix provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs.

miRNA linear target amplification over a dynamic range of 6 orders of magnitude of input

Amplification plot and standard curve from real-time PCR for a dilution series of a synthetic target amplified in four replicate reactions using the Applied Biosystems 7900HT Fast Real-Time PCR System and the Let7-c TaqMan MicroRNA Assay.

Amp plot and standard curve images for TaqMan Fast Advanced Master Mix

Complete your workflow

Maximize TaqMan real-time PCR results by combining TaqMan Fast Advanced Master Mix with TaqMan Gene Expression Assays and SuperScript IV VILO reverse transcription master mix. These high performing reagents were designed to work together to help accelerate your research by offering accurate, high-quality data at blazing fast speeds.

For Research Use Only. Not for use in diagnostic procedures.