Applied Biosystems™ TaqMan™ MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation and functional analysis. There are now even more choices for detection of mature, biologically active miRNA, designed to support a growing range of miRNA applications. The new Applied Biosystems™ TaqMan Advanced miRNA Assays use ligation-based universal reverse transcription for a streamlined and highly sensitive workflow. The standard TaqMan MicroRNA Assays, which employ target-specific stem-loop reverse transcription primers for 3’ extended templates, continue to cover a range of species using standard TaqMan Assay–based real-time PCR.
MicroRNA Analysis Using TaqMan Assays
TaqMan Advanced miRNA Assays on cards and plates
Be one of the first to use our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.
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New TaqMan Advanced miRNA Assays use universal reverse transcription and provide highly sensitive quantification.
Standard TaqMan MicroRNA Assays use a target-specific stem-loop reverse transcription primer.
Applied Biosystems™ TaqMan™ Pri-miRNA Assays detect and measure primary miRNAs from human, mouse, or rat species.
Assay search tool - find & buy your single tube TaqMan Assays:
miRBase v.21 update
The content for our standard TaqMan MicroRNA Assays and the new TaqMan Advanced miRNA Assays is aligned with release 21 of the miRBase database. For a complete list of standard TaqMan MicroRNA Assays, TaqMan Advanced miRNA Assays, and other technical resources, please visit our TaqMan MicroRNA Assays resource page.
Additional miRNA assays by type
Applied Biosystems™ TaqMan™ Non-coding RNA Assays for reliable detection and quantitation of non-coding human, mouse, or rat transcripts longer than 200 nucleotides.
Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.
miRNA assays by format
Order your TaqMan MicroRNA Assays preplated into Fast or standard 96-well plates with the Applied Biosystems™ TaqMan™ Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.
384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems™ ViiA™ 7 and 7900HT real-time PCR systems.
Order your TaqMan MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqMan Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or non-coding RNAs, or the converse, evaluating a few samples for a large number of targets.
These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems™ OpenArray™ Real-Time PCR System and the new Applied Biosystems™ QuantStudio™ 12K Flex Real-Time PCR System.
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Video: Find the right TaqMan MicroRNA Assay It's never been easier to search over 23,000 TaqMan MicroRNA Assays with the new Assay Search tool from Thermo Fisher Scientific. Watch this short video to see how easy it is to find the right TaqMan MicroRNA Assay. |
Resources
- Download your TaqMan documentation files
- Which TaqMan MicroRNA or Non-Coding RNA Assay is right for you?
- MicroRNA and non-coding RNA applications for qPCR
TaqMan MicroRNA Assays FAQs
Product Bulletin: Quantitate microRNAs with the specificity and sensitivity of real-time PCR
Download complete list of TaqMan MicroRNA Assays
New Protocol for generating Custom Pools of RT primers
Brochure: TaqMan microRNA Assays
For Research Use Only. Not for use in diagnostic procedures.